pCAG-TMPRSS2-3×FLAG plasmid

pCAG-TMPRSS2-3×FLAG plasmid

PVT89037

pCAG-TMPRSS2-3×FLAG plasmid Information

Plasmid name: pCAG-TMPRSS2(human)-3×FLAG
Alias: NM_001135099.1;PRSS10;
Gene lenght: 1587bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pcaggs-R
Copy number: High
Induction:

pCAG-TMPRSS2-3×FLAG plasmid Description

pCAG-TMPRSS2-3×FLAG is a plasmid suitable for protein expression in mammalian cells.

pCAG-TMPRSS2-3×FLAG plasmid Sequence

1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA 421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG 481 CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC 541 GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT 601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG 661 CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC 721 CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC 781 CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG 841 AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT 901 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC 961 GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG 1021 GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG 1081 TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC 1141 ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC 1201 GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG 1261 CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC 1321 GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG 1381 GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC 1441 ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG 1501 GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT 1561 CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT 1621 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG 1681 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGCCACC 1741 atgccccctg ccccgcccgg aggtgaaagc gggtgtgagg agcgcggcgc ggcaggtcat 1801 attgaacatt ccagatacct atcattactc gatgctgttg ataacagcaa gatggctttg 1861 aactcagggt caccaccagc tattggacct tactatgaaa accatggata ccaaccggaa 1921 aacccctatc ccgcacagcc cactgtggtc cccactgtct acgaggtgca tccggctcag 1981 tactacccgt cccccgtgcc ccagtacgcc ccgagggtcc tgacgcaggc ttccaacccc 2041 gtcgtctgca cgcagcccaa atccccatcc gggacagtgt gcacctcaaa gactaagaaa 2101 gcactgtgca tcaccttgac cctggggacc ttcctcgtgg gagctgcgct ggccgctggc 2161 ctactctgga agttcatggg cagcaagtgc tccaactctg ggatagagtg cgactcctca 2221 ggtacctgca tcaacccctc taactggtgt gatggcgtgt cacactgccc cggcggggag 2281 gacgagaatc ggtgtgttcg cctctacgga ccaaacttca tccttcaggt gtactcatct 2341 cagaggaagt cctggcaccc tgtgtgccaa gacgactgga acgagaacta cgggcgggcg 2401 gcctgcaggg acatgggcta taagaataat ttttactcta gccaaggaat agtggatgac 2461 agcggatcca ccagctttat gaaactgaac acaagtgccg gcaatgtcga tatctataaa 2521 aaactgtacc acagtgatgc ctgttcttca aaagcagtgg tttctttacg ctgtatagcc 2581 tgcggggtca acttgaactc aagccgccag agcaggattg tgggcggcga gagcgcgctc 2641 ccgggggcct ggccctggca ggtcagcctg cacgtccaga acgtccacgt gtgcggaggc 2701 tccatcatca cccccgagtg gatcgtgaca gccgcccact gcgtggaaaa acctcttaac 2761 aatccatggc attggacggc atttgcgggg attttgagac aatctttcat gttctatgga 2821 gccggatacc aagtagaaaa agtgatttct catccaaatt atgactccaa gaccaagaac 2881 aatgacattg cgctgatgaa gctgcagaag cctctgactt tcaacgacct agtgaaacca 2941 gtgtgtctgc ccaacccagg catgatgctg cagccagaac agctctgctg gatttccggg 3001 tggggggcca ccgaggagaa agggaagacc tcagaagtgc tgaacgctgc caaggtgctt 3061 ctcattgaga cacagagatg caacagcaga tatgtctatg acaacctgat cacaccagcc 3121 atgatctgtg ccggcttcct gcaggggaac gtcgattctt gccagggtga cagtggaggg 3181 cctctggtca cttcgaagaa caatatctgg tggctgatag gggatacaag ctggggttct 3241 ggctgtgcca aagcttacag accaggagtg tacgggaatg tgatggtatt cacggactgg 3301 atttatcgac aaatgagggc agacggcGGT ACCGACTACA AAGACCATGA CGGTGATTAT 3361 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT AGCTCGAGCT AGCAGATCTT 3421 TTTCCCTCTG CCAAAAATTA TGGGGACATC ATGAAGCCCC TTGAGCATCT GACTTCTGGC 3481 TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC 3541 GGAAGGACAT ATGGGAGGGC AAATCATTTA AAACATCAGA ATGAGTATTT GGTTTAGAGT 3601 TTGGCAACAT ATGCCCATAT GCTGGCTGCC ATGAACAAAG GTTGGCTATA AAGAGGTCAT 3661 CAGTATATGA AACAGCCCCC TGCTGTCCAT TCCTTATTCC ATAGAAAAGC CTTGACTTGA 3721 GGTTAGATTT TTTTTATATT TTGTTTTGTG TTATTTTTTT CTTTAACATC CCTAAAATTT 3781 TCCTTACATG TTTTACTAGC CAGATTTTTC CTCCTCTCCT GACTACTCCC AGTCATAGCT 3841 GTCCCTCTTC TCTTATGGAG ATCCCTCGAC CTGCAGCCCA AGCTTGGCGT AATCATGGTC 3901 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 3961 AAGCATAAAG TGTAAAGCCT GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG 4021 CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCGGATCCG CATCTCAATT 4081 AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT 4141 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG 4201 CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT 4261 GCAAAAAGCT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC 4321 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT 4381 CAATGTATCT TATCATGTCT GGATCCGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA 4441 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 4501 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 4561 TCAGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 4621 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 4681 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 4741 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 4801 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 4861 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 4921 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 4981 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 5041 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 5101 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 5161 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 5221 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 5281 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 5341 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 5401 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 5461 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 5521 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 5581 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 5641 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 5701 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 5761 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 5821 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 5881 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 5941 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 6001 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 6061 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 6121 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 6181 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 6241 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 6301 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 6361 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.