pLV3-CMV-Nectin2-3×FLAG-CopGFP

pLV3-CMV-Nectin2-3×FLAG-CopGFP-Puro plasmid

PVT90487

pLV3-CMV-Nectin2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Nectin2(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_008990.3;Cd112; MPH; nectin-2; Pvr; Pvrl2; Pvs
Gene lenght: 1590bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Nectin2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Nectin2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Nectin2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cccgggccgc agtcctcccg 2341 ccgtccagat tgtcaccgac gctgccgttg ttgccgctgc tactgctcct gcttcaggaa 2401 acaggagccc aagatgtgcg ggtacgagtg cttcccgagg tccggggccg cttgggaggc 2461 accgtggagt taccgtgcca cctgctccca cccacgacgg agcgcgtctc tcaggtgacc 2521 tggcagcgcc tggatggcac agttgtggct gctttccacc catccttcgg agtggatttc 2581 cccaactctc agttcagcaa ggaccgtctg tcctttgtca gagcgagacc agaaacaaac 2641 gcagacctgc gggatgccac actggccttc cggggactga gggtagagga cgagggcaat 2701 tacacctgcg agtttgccac gtttcccaac ggtacccgca ggggggtgac ctggctcaga 2761 gtcatagccc agcctgagaa ccacgctgaa gcccaggagg tcacaattgg cccccagtcg 2821 gtggctgtag cccgctgtgt ctccactggg ggccgccccc ctgcccgaat cacctggatc 2881 tcatctctgg gtggagaggc caaagatact caggagccag ggatacaggc tggcaccgtc 2941 actatcatca gccgatactc cttggtgccc gtgggccgag cggatggcgt caaggtcacg 3001 tgtagagtgg aacacgagag cttcgaagag ccgatcctgc tgccagtgac cctctctgtg 3061 cgctaccctc cagaagtatc catctccggc tatgatgaca actggtacct tggccgcagt 3121 gaggccatac tgacctgtga tgtacgaagc aacccagagc ccacagacta tgactggagc 3181 acgacctcgg gcgtcttccc agcctctgca gtggcccagg gctctcagct gcttgtccac 3241 tctgtggatc gaatggtcaa cactaccttc atctgtacag ccaccaacgc tgtggggaca 3301 ggccgtgctg agcaggtcat cctggtgcga gagtcaccca gcacagcagg agcaggggcc 3361 actggtggca tcattggagg tattatcgct gccatcatcg ccaccgcagt ggctggcaca 3421 ggcatcctca tctgccgaca acagcggaag gagcagaggc ttcaagctgc ggatgaggaa 3481 gaagaactgg aaggacctcc ctcctataaa ccacccaccc cgaaggccaa gctggaggaa 3541 ccagagatgc cctctcaact cttcaccttg ggggcctcag agcacagccc agtgaagacg 3601 ccatactttg atgctggtgt ctcttgtgct gatcaggaga tgcctcggta tcacgagctg 3661 cccactctgg aagagcggtc agggcccctg ctgttggggg ctacaggcct gggaccttct 3721 cttctggtgc ctccaggacc caatgttgtg gagggggttt ccctgagtct cgaagatgag 3781 gaggaagatg atgaggagga agacttcctg gataaaatca accctattta tgatgccctg 3841 tcctacccca gcccctctga ctcctaccag agcaaagact tttttgtgtc acgggccatg 3901 tatgtgggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 3961 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 4021 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 4081 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 4141 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 4201 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 4261 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 4321 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 4381 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 4441 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 4501 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 4561 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 4621 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 4681 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4741 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4801 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4861 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4921 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 4981 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 5041 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 5101 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 5161 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 5221 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 5281 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 5341 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 5401 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 5461 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 5521 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5581 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5641 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5701 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5761 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5821 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5881 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5941 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 6001 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 6061 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 6121 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 6181 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 6241 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 6301 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 6361 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6421 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6481 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 6541 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6601 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 6661 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6721 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6781 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6841 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6901 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6961 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 7021 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 7081 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 7141 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 7201 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 7261 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 7321 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 7381 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 7441 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7501 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7561 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7621 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7681 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 8041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 8101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 8161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 8221 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 8281 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 8341 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 8401 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 8461 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 8521 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8581 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8641 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8701 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8761 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8821 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8881 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8941 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 9001 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 9061 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 9121 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 9181 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 9241 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 9301 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 9361 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 9421 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 9481 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 9541 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9601 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9661 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9841 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.