pLV3-CMV-MCS-3×HA-mCherry-Hyg

pLV3-CMV-MCS-3×HA-mCherry-Hyg plasmid

PVT90802

pLV3-CMV-MCS-3×HA-mCherry-Hyg plasmid Information

Plasmid name: pLV3-CMV-MCS-3×HA-mCherry-Hyg
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-MCS-3×HA-mCherry-Hyg plasmid Description

pLV3-CMV-MCS-3×HA-mCherry-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MCS-3×HA-mCherry-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcC TTAAGCTTGG TACCGAGCTC GAATTCCTCG AGgccaccat gTACCCATAC 2341 GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT 2401 GATGTCCCAG ACTACGCATA GgcggccgcG aaggatctgc gatcgctccg gtgcccgtca 2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 2521 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 2641 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc 2701 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct 2761 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct 2821 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct 2881 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct 2941 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccaccA TGGTGAGCAA 3001 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA 3061 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA 3121 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA 3181 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT 3241 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT 3301 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT 3361 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA 3421 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG 3481 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC 3541 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT 3601 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG 3661 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGTC GAGGGAAGCG GAGCTACTAA 3721 CTTCAGCCTG CTGAAGCAGG CTGGAGACGT GGAGGAGAAC CCTGGACCTG TCGACatgaa 3781 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt 3841 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg 3901 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta 3961 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga 4021 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga 4081 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat 4141 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg 4201 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg 4261 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat 4321 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa 4381 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt 4441 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat 4501 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct 4561 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa 4621 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg 4681 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt 4741 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata 4801 gTCTAGAaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 4861 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 4921 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 4981 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 5041 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 5101 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 5161 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaATCATCGT CCTTTCCTtg 5221 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 5281 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 5341 gcgtctccgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 5401 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 5461 aggggggact ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta 5521 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 5581 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 5641 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 5701 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 5761 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 5821 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 5881 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgccATCCCG 5941 CCCTAACTCC GCcagttccg cccattctcc gccccatggc tgactaattt tttttattta 6001 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 6061 tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc 6121 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 6181 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 6241 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 6301 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 6361 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 6421 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6481 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6541 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6601 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6661 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 6721 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6781 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6841 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6901 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6961 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 7021 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 7081 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 7141 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 7201 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 7261 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 7321 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 7381 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 7441 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7501 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7561 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7621 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7681 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7741 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7801 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7861 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 7921 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7981 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 8041 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 8101 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 8161 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 8221 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 8281 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 8341 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 8401 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 8461 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 8521 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 8581 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 8641 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 8701 acgttgtaaa acgacggcca gtgccaagct g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.