pLV3-CMV-SLC7A5-3×Myc-Blast pl

pLV3-CMV-SLC7A5-3×Myc-Blast plasmid

PVT94581

pLV3-CMV-SLC7A5-3×Myc-Blast plasmid Information

Plasmid name: pLV3-CMV-SLC7A5(human)-3×Myc-Blast
Alias: NM_003486.7;4F2LC; CD98; D16S469E; E16; LAT1; MPE16
Gene lenght: 1521bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-SLC7A5-3×Myc-Blast plasmid Description

pLV3-CMV-SLC7A5-3×Myc-Blast is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-SLC7A5-3×Myc-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggcgggt gcgggcccga agcggcgcgc gctagcggcg 2341 ccggcggccg aggagaagga agaggcgcgg gagaagatgc tggccgccaa gagcgcggac 2401 ggctcggcgc cggcaggcga gggcgagggc gtgaccctgc agcggaacat cacgctgctc 2461 aacggcgtgg ccatcatcgt ggggaccatt atcggctcgg gcatcttcgt gacgcccacg 2521 ggcgtgctca aggaggcagg ctcgccgggg ctggcgctgg tggtgtgggc cgcgtgcggc 2581 gtcttctcca tcgtgggcgc gctctgctac gcggagctcg gcaccaccat ctccaaatcg 2641 ggcggcgact acgcctacat gctggaggtc tacggctcgc tgcccgcctt cctcaagctc 2701 tggatcgagc tgctcatcat ccggccttca tcgcagtaca tcgtggccct ggtcttcgcc 2761 acctacctgc tcaagccgct cttccccacc tgcccggtgc ccgaggaggc agccaagctc 2821 gtggcctgcc tctgcgtgct gctgctcacg gccgtgaact gctacagcgt gaaggccgcc 2881 acccgggtcc aggatgcctt tgccgccgcc aagctcctgg ccctggccct gatcatcctg 2941 ctgggcttcg tccagatcgg gaagggtgat gtgtccaatc tagatcccaa cttctcattt 3001 gaaggcacca aactggatgt ggggaacatt gtgctggcat tatacagcgg cctctttgcc 3061 tatggaggat ggaattactt gaatttcgtc acagaggaaa tgatcaaccc ctacagaaac 3121 ctgcccctgg ccatcatcat ctccctgccc atcgtgacgc tggtgtacgt gctgaccaac 3181 ctggcctact tcaccaccct gtccaccgag cagatgctgt cgtccgaggc cgtggccgtg 3241 gacttcggga actatcacct gggcgtcatg tcctggatca tccccgtctt cgtgggcctg 3301 tcctgcttcg gctccgtcaa tgggtccctg ttcacatcct ccaggctctt cttcgtgggg 3361 tcccgggaag gccacctgcc ctccatcctc tccatgatcc acccacagct cctcaccccc 3421 gtgccgtccc tcgtgttcac gtgtgtgatg acgctgctct acgccttctc caaggacatc 3481 ttctccgtca tcaacttctt cagcttcttc aactggctct gcgtggccct ggccatcatc 3541 ggcatgatct ggctgcgcca cagaaagcct gagcttgagc ggcccatcaa ggtgaacctg 3601 gccctgcctg tgttcttcat cctggcctgc ctcttcctga tcgccgtctc cttctggaag 3661 acacccgtgg agtgtggcat cggcttcacc atcatcctca gcgggctgcc cgtctacttc 3721 ttcggggtct ggtggaaaaa caagcccaag tggctcctcc agggcatctt ctccacgacc 3781 gtcctgtgtc agaagctcat gcaggtggtc ccccaggaga caggtggagg cggatcagga 3841 ggcggtggat ctCTCGAGGA ACAAAAACTA ATATCAGAGG AAGATTTGGA ACAGAAACTA 3901 ATCAGTGAAG AAGATTTAGA GCAAAAGTTA ATTTCCGAGG AGGACTTATA GGCGGCCGCG 3961 AAGGATCTGC GATCGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC 4021 CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACGGGTGCC TAGAGAAGGT GGCGCGGGGT 4081 AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC 4141 GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC 4201 ACAGCTGAAG CTTCGAGGGG CTCGCATCTC TCCTTCACGC GCCCGCCGCC CTACCTGAGG 4261 CCGCCATCCA CGCCGGTTGA GTCGCGTTCT GCCGCCTCCC GCCTGTGGTG CCTCCTGAAC 4321 TGCGTCCGCC GTCTAGGTAA GTTTAAAGCT CAGGTCGAGA CCGGGCCTTT GTCCGGCGCT 4381 CCCTTGGAGC CTACCTAGAC TCAGCCGGCT CTCCACGCTT TGCCTGACCC TGCTTGCTCA 4441 ACTCTACGTC TTTGTTTCGT TTTCTGTTCT GCGCCGTTAC AGATCCAAGC TGTGACCGGC 4501 GCCTACGCTA GGCCACCATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA 4561 GAGCAACGGC TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG 4621 CTCTCTCTAG CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC 4681 CTTGTGCAGA ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT 4741 GTATCGTCGC GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC 4801 AGGTGCTTCT CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC 4861 CGACGGCAGT TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAAGTCG 4921 ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG 4981 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC 5041 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT 5101 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA 5161 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC 5221 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC 5281 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC 5341 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC 5401 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC 5461 TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGTACCT 5521 TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG 5581 GGACTGGAAG GGCTAATTCA CTCCCAACGA AAATAAGATC TGCTTTTTGC TTGTACTGGG 5641 TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG 5701 CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT 5761 GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT 5821 AGTAGTTCAT GTCATCTTAT TATTCAGTAT TTATAACTTG CAAAGAAATG AATATCAGAG 5881 AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA 5941 AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC 6001 AATGTATCTT ATCATGTCTG GCTCTAGCTA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 6061 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG 6121 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGAC TTTTGCAGAG 6181 ACGGCCCAAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 6241 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 6301 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 6361 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 6421 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 6481 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 6541 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 6601 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 6661 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 6721 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 6781 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 6841 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 6901 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 6961 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 7021 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 7081 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 7141 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 7201 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 7261 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 7321 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 7381 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 7441 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 7501 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 7561 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 7621 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 7681 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 7741 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 7801 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 7861 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 7921 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 7981 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 8041 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 8101 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 8161 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 8221 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 8281 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 8341 GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA 8401 GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 8461 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 8521 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 8581 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA 8641 GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC 8701 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC 8761 GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG 8821 CCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.