pAdTrack-HPV18-E5 plasmid

pAdTrack-HPV18-E5 plasmid

PVT89703

pAdTrack-HPV18-E5 plasmid Information

Plasmid name: pAdTrack-HPV18-E5(virus)
Alias:
Gene lenght: 222bp
Host: Mammalian cells,Adenovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Virus
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: Non
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: Sv40-polyA-R
Copy number: High
Induction:

pAdTrack-HPV18-E5 plasmid Description

pAdTrack-HPV18-E5 This is a plasmid suitable for protein expression in mammalian cells and can also be used for adenovirus packaging.

pAdTrack-HPV18-E5 plasmid Sequence

1 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 61 nnnncggcgc attaagcgcg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 121 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 181 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gagctttacg 241 gcacctcgac cgcaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg 301 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 361 ccaaactgga acaacactca accctatcgc ggtctattct tttgatttat aagggatgtt 421 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaatttt aacaaaattc 481 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac 541 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg 601 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc 661 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga 721 cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg gctggcgcga 781 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac 841 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg 901 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag 961 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag 1021 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg 1081 ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg 1141 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg 1201 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat 1261 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagagctt gatcccctgc 1321 gccatcagat ccttggcggc gagaaagcca tccagtttac tttgcagggc ttcccaacct 1381 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt 1441 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt 1501 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac 1561 tggctttcta cgtgaaaagg atctaggtga agatcctttt tnnnnnncaa caacgttgcg 1621 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 1681 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 1741 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 1801 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 1861 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 1921 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 1981 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 2041 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 2101 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 2161 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 2221 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 2281 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 2341 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 2401 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 2461 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 2521 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 2581 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 2641 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 2701 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 2761 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 2821 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct 2881 tacgcatctg tgcggtattt cacaccgnnn ggatccttaa ttaannnatc atcaataata 2941 taccttattt tggattgaag ccaatatgat aatgaggggg tggagtttgt gacgtggcgc 3001 ggggcgtggg aacggggcgg gtgacgtagg ttttagggcg gagtaacttg tatgtgttgg 3061 gaattgtagt tttcttaaaa tgggaagtta cgtaacgtgg gaaaacggaa gtgacgattt 3121 gaggaagttg tgggtttttt ggctttcgtt tctgggcgta ggttcgcgtg cggttttctg 3181 ggtgtttttt gtggacttta accgttacgt cattttttag tcctatatat actcgctctg 3241 cacttggccc ttttttacac tgtgactgat tgagctggtg ccgtgtcgag tggtgttttt 3301 ttaataggtt ttctttttta ctggtaaggc tgactgttag gctgccgctg tgaagcgctg 3361 tatgttgttc tggagcggga gggtgctatt ttgcctaggc aggagggttt ttcaggtgtt 3421 tatgtgtttt tctctcctat taattttgtt atacctccta tgggggctgt aatgttgtct 3481 ctacgcctgc gggtatgtat tcccccgggc tatttcggtc gctttttagc actgaccgat 3541 gaatcaacct gatgtgttta ccgagtctta cattatgact ccggacatga ccgaggagct 3601 gtcggtggtg ctttttaatc acggtgacca gtttttttac ggtcacgccg gcatggccgt 3661 agtccgtctt atgcttataa gggttgtttt tcctgttgta agacaggctt ctaatgttta 3721 aatgtttttt tgttatttta ttttgtgttt atgcagaaac ccgcagacat gtttgagaga 3781 aaaatggtgt ctttttctgt ggtggttccg gagcttacct gcctttatct gcatgagcat 3841 gactacgatg tgctttcttt tttgcgcgag gctttgcctg attttttgag cagcaccttg 3901 cattttatan nngaattcgt ttaaacnnna gtctgtatac ggggacacgg acagcctttt 3961 cgtcaccgag cgtggacacc ggctcatgga aaccagaggt aagaaacgca tcaaaaagca 4021 tgggggaaac ctggtttttg accccgaacg gccagagctc acctggctcg tggaatgcga 4081 gaccgtctgc ggggcctgcg gcgcggatgc ctactccccg gaatcggtat ttctcgcgcc 4141 caagctctac gccctcaaaa gtctgcactg cccctcgtgc ggcgcctcct ccaagggcaa 4201 gctgcgcgcc aagggccacg ccgcggaggg gctggactat gacaccatgg tcaaatgcta 4261 cctggccgac gcgcagggcg aagaccggca gcgcttcagc accagcagga ccagcctcaa 4321 gcgcaccctg gccagcgcgc agcccggagc gcaccccttc accgtgaccc agactacgct 4381 gacgaggacc ctgcgcccgt ggaaagacat gaccctggcc cgtctggacg agcaccgact 4441 actgccgtac agcgaaagcc gccccaaccc gcgaaacgag gagatatgct ggatcgagat 4501 gccgtagagc aggtgaccga gctgtgggac cgcctggaac tgcttggtca aacgctcaaa 4561 agcatgccta cggcggacgg tctcaaaccg ttgaaaaact ttgcttcctt gcaagaactg 4621 ctatcgctgg gcggcgagcg ccttctggcg gatttggtca gggaaaacat gcgagtcagg 4681 gacatgctta acgaagtggc ccccctgctc agggatgacg gcagctgcag ctctcttaac 4741 taccagttgc acccggtaat aggtgtgatt tacgggccca ccggctgcgg taagtcgcag 4801 ctgctcagga acctgctttc ttcccagctg atctccccta ccccggaaac cgttttcttc 4861 atcgccccgc aggtagacat gatcccccca tctgaactca aagcgtggga aatgcaaatc 4921 tgtgagggta actacgcccc tgggccggat ggaaccatta taccgcagtc tggcaccctc 4981 cgcccgcgct ttgtaaaaat ggcctatgac gatctcatcc tggaacacaa ctatgacgtt 5041 agtgatccca gaaatatctt cgcccaggcc gccgcccgtg ggcccattgc catcattatg 5101 gacgaatgca tggaaaatct tggaggtcac aagggcgtct ccaagttctt ccacgcattt 5161 ccttctaagc tacatgacaa atttcccaag tgcaccggat acactgtgct ggtggttctg 5221 cacaacatga atccccggag ggatatggct gggaacatag ccaacctaaa aatacagtcc 5281 aagatgcatc tcatatcccc acgtatgcac ccatcccagc ttaaccgctt tgtaaacact 5341 tacaccaagg gcctgcccct ggcaatcagc ttgctactga aagacatttt taggcaccac 5401 gcccagcgct cctgctacga ctggatcatc tacaacacca ccccgcagca tgaagctctg 5461 cagtggtgct acctccaccc cagagacggg cttatgccca tgtatctgaa catccagagt 5521 cacctttacc acgtcctgga aaaaatacac aggaccctca acgaccgaga ccgctggtcc 5581 cgggcctacc gcgcgcgcaa aacccctaaa taaagacagc aagacacttg cttgatccaa 5641 atccaaacag agtctggttt tttatttatg ttttaaaccg cattgggagg ggaggaagcc 5701 ttcagggcag aaacctgctg gcgcagatcc aacagctgct gagaaacgac attaagttcc 5761 cgggtcaaag aatccaattg tgccaaaaga gccgtcaact tgtcatcgcg ggcggatgaa 5821 cgggaagctg cactgcttgc aagcgggctc aggaaagcaa agtcagtcac aatcccgcgg 5881 gcggtggctg cagcggctga agcggcggcg gaggctgcag tctccaacgg cgttccagac 5941 acggtctcgt aggtcaaggt agtagagttt gcgggcagga cggggcgacc atcaatgctg 6001 gagcccatca cattctgacg caccccggcc catgggggca tgcgcgttgt caaatatgag 6061 ctcacaatgc ttccatcaaa cgagttggtg ctcatggcgg cggcggctgc tgcaaaacag 6121 atacaaaact acataagacc cccaccttat atattctttc ccacccttaa ccacgcccag 6181 atcctctaga aagcttatgt tatcacttat ttttttattt tgcttttgtg tatgcatgta 6241 tgtgtgctgc catgtcccgc ttttgccatc tgtctgtatg tgtgcgtatg catgggtatt 6301 ggtatttgtg tatattgtgg taataacgtc ccctgccaca gcattcacag tatatgtatt 6361 ttgtttttta ttgcccatgt tactattgca tatacatgct atattgtctt tacagtaagt 6421 cgacggtacc agatctaata gtaatcaatt acggggtcat tagttcatag ccaacttgtt 6481 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 6541 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgA 6601 TCAAGCTAGC TTGCTAGACT CGACTGACTA TAATAATAAA ACGCCAACTT TGACCCGGAA 6661 CGCGGAAAAC ACCTGAGAAA AACACCTGGG CGAGTCTCCA CGTAAACGGT CAAAGTCCCC 6721 GCGGCCCTAG ACAAATAtta cgcgctatga gtaacacaaa attattcaga tttcacttcc 6781 tcttattcag ttttcccgcg aaaatggcca aatcttactc ggttacgccc aaatttacta 6841 caacatccgc ctaaaaccgc gcgaaaattg tcacttcctg tgtacaccgg cgcacaccaa 6901 aaacgtcact tttgccacat ccgtcgctta catgtgttcc gccacacttg caacatcaca 6961 cttccgccac actactacgt cacccgcccc gttcccacgc cccgcgccac gtcacaaact 7021 ccaccccctc attatcatat tggcttcaat ccaaaagggg cagagagctg gaagggannn 7081 ttaattaan

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.