pLV3-CMV-NXPH4-3×FLAG-CopGFP-P

pLV3-CMV-NXPH4-3×FLAG-CopGFP-Puro plasmid

PVT89590

pLV3-CMV-NXPH4-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-NXPH4(human)-3×FLAG-CopGFP-Puro
Alias:
Gene lenght: 924bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NXPH4-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-NXPH4-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-NXPH4-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGC GGCTGCTCCC GGAATGGTTC 2341 CTCTTGCTCT TTGGCCCGTG GCTCCTTAGG AAGGCCGTCA GTGCCCAGAT ACCAGAGTCC 2401 GGAAGGCCGC AGTACCTGGG GCTGCGCCCC GCCGCGGCCG GAGCGGGTGC CCCCGGCCAG 2461 CAGCTCCCAG AGCCAAGGTC TTCGGACGGC CTAGGCGTGG GCCGCGCCTG GAGCTGGGCC 2521 TGGCCGACCA ACCACACGGG GGCGCTGGCC CGGGCAGGGG CAGCCGGGGC GTTGCCCGCG 2581 CAGCGCACCA AGAGGAAGCC GTCCATCAAG GCGGCGCGCG CCAAAAAGAT CTTCGGCTGG 2641 GGGGACTTCT ACTTTCGGGT GCATACCCTC AAGTTTTCGC TGCTGGTGAC CGGCAAGATC 2701 GTGGACCATG TGAACGGTAC CTTCAGTGTG TATTTCCGCC ACAACTCGTC CAGCCTGGGC 2761 AACCTCAGTG TCAGCATCGT GCCGCCCTCC AAGCGTGTCG AGTTCGGAGG AGTCTGGCTG 2821 CCCGGGCCTG TCCCCCACCC TCTGCAGTCT ACGCTCGCCC TGGAGGGGGT GCTTCCTGGG 2881 CTGGGGCCCC CGCTGGGGAT GGCAGCAGCA GCGGCGGGGC CCGGGCTTGG GGGCTCCCTC 2941 GGGGGCGCAC TGGCGGGGCC GCTTGGGGGC GCGTTGGGAG TGCCTGGGGC CAAAGAGTCA 3001 CGCGCTTTCA ATTGCCACGT GGAGTATGAG AAGACAAACC GCGCGCGCAA GCACCGACCG 3061 TGCCTGTACG ACCCGTCGCA GGTGTGCTTC ACCGAGCACA CGCAGAGCCA GGCCGCCTGG 3121 CTCTGTGCCA AGCCCTTCAA AGTCATCTGT ATCTTCGTCT CTTTCCTCAG CTTTGACTAC 3181 AAACTGGTGC AGAAGGTGTG CCCAGACTAT AACTTCCAGA GTGAGCACCC CTACTTCGGA 3241 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 3301 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 3361 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 3421 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 3481 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 3541 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 3601 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 3661 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 3721 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 3781 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 3841 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 3901 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 3961 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 4021 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 4081 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 4141 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 4201 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 4261 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 4321 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 4381 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 4441 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 4501 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 4561 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 4621 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 4681 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 4741 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 4801 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 4861 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 4921 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 4981 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 5041 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 5101 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 5161 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 5221 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 5281 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 5341 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 5401 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 5461 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 5521 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 5581 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 5641 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 5701 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 5761 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 5821 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 5881 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 5941 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 6001 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 6061 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 6121 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 6181 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 6241 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 6301 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 6361 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 6421 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6481 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 6541 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 6601 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 6661 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 6721 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 6781 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 6841 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 6901 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 6961 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7021 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7081 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7141 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7201 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7261 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7321 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7381 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 7441 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7501 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7561 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7621 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 7681 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 7741 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 7801 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 7861 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 7921 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 7981 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 8041 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 8101 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 8161 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 8221 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 8281 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 8341 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 8401 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 8461 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 8521 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 8581 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 8641 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 8701 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 8761 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 8821 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 8881 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 8941 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 9001 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 9061 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 9121 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 9181 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.