pLV3-CMV-Met-EGFP-3×FLAG-Blast

pLV3-CMV-Met-EGFP-3×FLAG-Blast plasmid

PVT91245

pLV3-CMV-Met-EGFP-3×FLAG-Blast plasmid Information

Plasmid name: pLV3-CMV-Met(rat)-EGFP-3×FLAG-Blast
Alias: NM_031517.3;Hgfr
Gene lenght: 4146bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-Met-EGFP-3×FLAG-Blast plasmid Description

pLV3-CMV-Met-EGFP-3×FLAG-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Met-EGFP-3×FLAG-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgaaggct cccaccgcgc tggcacctgg cattctgctg 2341 ctgctgctga ccttggcgca gaggagccat ggggagtgca aggaggccct agtgaagtct 2401 gagatgaacg tgaacatgaa gtaccagctt cccaacttca ccgcagaaac ccccatccac 2461 aatgtcgtcc tccatgggca ccatatttat ctcggagcca caaactacat ttatgtttta 2521 gatgacaaag accttcagaa ggtatctgag ttcaagaccg ggcccgtggt ggaacaccca 2581 gattgttttc cttgtcagga ctgcagcagc aaagccaatg tgtcaggagg tgtttggaaa 2641 gacaacgtca acatggcgct gcttgttgac acttactatg acgaccagct catcagctgt 2701 ggcagcgtca acagagggac ctgccaaagg catgtccttc ctcctgacaa tgctgccgac 2761 attcagtccg aggttcactg catgttctcc ccacttgcgg aggaagagtc aggccagtgt 2821 cccgactgtg tagtgagtgc cctgggagcc aaagtcctcc tgtctgaaaa ggaccggttc 2881 atcaatttct tcgtggggaa tacgataaac tcttcctacc ctcccgatta ttcattgcat 2941 tcaatatcgg tgaggcggct gaaggaaacc caggacggtt ttaagttttt gacagaccag 3001 tcctacattg atgtcctgcc agaattccga gattcctacc ccatcaagta catacatgcc 3061 ttcgaaagca accattttat ctactttctg actgtccaga aggaaaccct agatgctcag 3121 actttccata caagaataat caggttctgt tctgtagact ctgggttgca ctcctacatg 3181 gaaatgcctc tggagtgcat tctgacggaa aaaagaagaa agagatccac aagggaagaa 3241 gtgtttaata tcctccaagc cgcatatgtc agtaaaccgg gggccaatct tgctaagcaa 3301 ataggggcca gcccgtatga tgacattctc tacggggtgt ttgcacaaag caagccagat 3361 tctgctgagc ccatgaaccg atcagcggtc tgtgcattcc ccatcaaata tgtcaatgac 3421 ttcttcaaca agattgtcaa caaaaacaac gtacggtgtc tccagcattt ttatggaccc 3481 aaccacgagc actgtttcaa taggaccctg ctgagaaatt catcgggctg cgaagtgcgc 3541 agtgacgagt accggacgga gtttaccacg gcgctgcagc gtgtggattt attcatgggc 3601 cggctcaacc atgtactctt gacgtctatc tctaccttca tcaaaggtga cctcaccatt 3661 gctaatctag ggacatcaga aggtcgcttc atgcaggtgg tgctctctcg cacagcacat 3721 ttcacccccc atgtgaattt cctcctggat tcctatcctg tgtctccgga agttattgtc 3781 gaacatccat caaatcaaaa tggctatacc ttggtggtca cagggaagaa gatcaccaag 3841 attccactga atggcctagg ctgtgggcat ttccagtcct gcagtcagtg tctctctgcc 3901 ccctacttta tacagtgtgg ctggtgccac aatcggtgtg tgcattccaa tgaatgcccc 3961 agcggtacat ggactcaaga gatctgtctg ccagcagttt ataaggtttt ccccactagt 4021 gcacccctcg aaggaggaac aatgctgacc atatgtggct gggactttgg attcaagaag 4081 aataataaat ttgatttaag gaaaaccaaa gttctgcttg gcaacgagag ctgtaccttg 4141 accttaagcg agagcacgac aaatacgttg aaatgcacag ttggccccgc gatgagtgag 4201 cacttcaatg tgtctgtgat cgtctcaaac agtcgagaga caacacagta cagtgcgttt 4261 tcctatgtgg atcctgtaat aacaagtatt tctccaaggt atggtcctca tgccggaggc 4321 accttactca ctttgactgg aaaatacctc aacagcggca attctagaca catttcaatc 4381 ggagggaaaa catgtacttt aaaaagtgta tcagatagca ttctcgaatg ctacacccca 4441 ggccacaccg tctctgccga gtttcccgtg aaattgaaaa tcgacctggc tgaccgagtg 4501 acaagcagct tcagttaccg ggaagacccg gttgtctctg aaatccaccc gaccaaatct 4561 tttatcagtg gtggaagcac aataacgggg attggaaaga acctgaattc agttagcacc 4621 ccaaagctgg taatagaagt gcatgacgtg ggcgtgaact acaccgtggc gtgccaacat 4681 cgctcgagtt cagagatcat ctgctgcacc actccttccc tgcaacagct ggacctgcaa 4741 ctccccctga agaccaaagc cttcttcctg ctggacggga tcctttccaa acactttgat 4801 ctcacttatg tacatgatcc tatgtttaag ccttttgaaa agccagtaat gatctccatg 4861 ggcaatgaga atgtagtgga aattaaggga gacgatattg accctgaagc agttaaaggt 4921 gaagtgttaa aagtcgggaa taagagctgt gagaatctcc actggcattc tgaagctttg 4981 ttgtgtacgg tccccagtga cctgctgaag ctgaacggcg gcgagctaaa tatagagtgg 5041 aagcaagcag tctcttcaac tgtccttgga aaagtgatcg ttcaaccgga tcagaatttt 5101 gcaggattga tcattggtgc ggtctcaata tcagtggtag ttttgttagt atccgggctc 5161 ttcctgtggc tgagaaagag aaagcataaa gatctgggca gtgaattagt tcgctatgac 5221 gcaagagtac acactcctca tttggatagg cttgtaagtg cccgaagtgt aagcccaact 5281 acagagatgg tctcaaatga gtctgtagac tacagagcta cttttccaga agaccagttt 5341 cccaactcct ctcagaatgg agcctgcaga caagtgcagt atcctctgac agatctgtcc 5401 cccatcctga cgagtggaga ctctgatata tccagcccat tactacaaaa cactgttcac 5461 attgacctca gcgctctaaa tccagagctg gtccaagcgg tgcagcacgt agtgattgga 5521 cccagtagcc tgattgtgca tttcaatgaa gtcataggaa gagggcattt tggctgtgtc 5581 tatcatggga ctttgttgga cagtgacgga aagaaaattc actgtgctgt gaaatccttg 5641 aacagaatca cagatataga agaagtctcc cagtttctga ctgagggaat catcatgaaa 5701 gatttcagcc accccaatgt tctctcactc ttgggaatct gcctgcggag tgaagggtcc 5761 cctctggtgg ttctgcccta tatgaagcac ggagatcttc gcaatttcat tcgaaatgag 5821 actcataacc caactgtgaa agatcttata ggattcggtc ttcaagtagc caagggcatg 5881 aaatatcttg ccagcaaaaa gtttgtccac agagacttag ctgcaagaaa ctgcatgttg 5941 gatgaaaaat tcactgtcaa ggttgctgat ttcggtcttg ccagagacat gtacgacaaa 6001 gagtattata gcgtccacaa caaaacgggt gcgaaactac cggtgaagtg gatggcttta 6061 gagagtctgc agacgcaaaa gttcaccacc aagtcagacg tgtggtcctt cggtgtgctt 6121 ctctgggagc tcatgacgag aggagcccct ccttatcctg acgtgaacac atttgatatc 6181 actatatacc tgttgcaagg cagaagactc ttgcaaccag agtactgtcc agacgccttg 6241 tatgaagtga tgctaaaatg ctggcacccc aaagcagaaa tgcgcccatc gttttctgaa 6301 ctggtctcca ggatatcctc aatcttctcc actttcattg gcgagcacta tgtccatgtg 6361 aacgctactt atgtgaatgt aaaatgtgtt gctccatatc cttctctgtt gccatcccaa 6421 gacaacattg acggcgaagc gaacacaGGC GGTGGAGGAA GCggcggagg tggatctatg 6481 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 6541 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 6601 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 6661 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 6721 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 6781 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 6841 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 6901 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 6961 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 7021 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 7081 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 7141 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagGGATCC 7201 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 7261 GACAAGtaGC GGCCGCGAAG GATCTGCGAT CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC 7321 ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC GGGTGCCTAG 7381 AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC 7441 GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC 7501 GGGTTTGCCG CCAGAACACA GCTGAAGCTT CGAGGGGCTC GCATCTCTCC TTCACGCGCC 7561 CGCCGCCCTA CCTGAGGCCG CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC 7621 TGTGGTGCCT CCTGAACTGC GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG 7681 GGCCTTTGTC CGGCGCTCCC TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC 7741 CTGACCCTGC TTGCTCAACT CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA 7801 TCCAAGCTGT GACCGGCGCC TACGCTAGGC CACCATGGCC AAGCCTTTGT CTCAAGAAGA 7861 ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT CTGAAGACTA 7921 CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG TCAATGTATA 7981 TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG CTGCTGCGGC 8041 AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA TCTTGAGCCC 8101 CTGCGGACGG TGCCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG CCATAGTGAA 8161 GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT CTGGTTATGT 8221 GTGGGAGGGC TAAGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 8281 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 8341 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 8401 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 8461 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 8521 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 8581 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC 8641 GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 8701 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 8761 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 8821 CTCCCCGCCT GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT 8881 TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAAA TAAGATCTGC 8941 TTTTTGCTTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT 9001 AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT 9061 GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT 9121 GGAAAATCTC TAGCAGTAGT AGTTCATGTC ATCTTATTAT TCAGTATTTA TAACTTGCAA 9181 AGAAATGAAT ATCAGAGAGT GAGAGGAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 9241 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 9301 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGCT CTAGCTATCC CGCCCCTAAC 9361 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA 9421 GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG 9481 CCTAGACTTT TGCAGAGACG GCCCAAATTC GTAATCATGG TCATAGCTGT TTCCTGTGTG 9541 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 9601 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 9661 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 9721 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 9781 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 9841 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 9901 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 9961 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 10021 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 10081 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 10141 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 10201 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 10261 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 10321 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 10381 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 10441 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 10501 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 10561 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 10621 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 10681 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 10741 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 10801 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 10861 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 10921 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 10981 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 11041 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 11101 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 11161 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 11221 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 11281 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 11341 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 11401 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 11461 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 11521 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 11581 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 11641 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC 11701 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 11761 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 11821 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 11881 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 11941 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG 12001 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG 12061 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG 12121 TAAAACGACG GCCAGTGCCA AGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.