pCMV-HA-TULP2 plasmid

pCMV-HA-TULP2 plasmid

PVT89270

pCMV-HA-TULP2 plasmid Information

Plasmid name: pCMV-HA-TULP2(human)
Alias: NM_003323.3;CT65; TUBL2
Gene lenght: 1560bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: SV40 intron-F1
Reverse primer:
Copy number: High
Induction:

pCMV-HA-TULP2 plasmid Description

pCMV-HA-TULP2 is a plasmid suitable for protein expression in mammalian cells.

pCMV-HA-TULP2 plasmid Sequence

1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG 121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG 181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA 241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA 541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC 601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG 661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG 721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA 781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GTACCCATAC 841 GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA TTCGCGCCAC CATGTCTCAG 901 GATAATGACA CATTGATGAG AGACATCCTG GGGCATGAGC TCGCTGCTAT GAGGCTGCAG 961 AAGCTGGAAC AGCAGCGGCG GCTGTTTGAA AAGAAGCAGC GACAGAAGCG CCAGGAGCTC 1021 CTCATGGTTC AGGCCAATCC TGACGCTTCC CCGTGGCTTT GGCGCTCTTG TCTGCGGGAG 1081 GAGCGCCTTT TAGGTGACAG AGGCCTTGGG AACCCTTTCC TCCGGAAGAA AGTGTCAGAG 1141 GCACATCTGC CCTCTGGCAT CCACAGTGCC CTGGGCACCG TGAGCTGTGG TGGAGACGGC 1201 AGGGGCGAGC GCGGCCTCCC GACACCGCGG ACAGAAGCAG TGTTCAGGAA TCTCGGTCTC 1261 CAGTCCCCTT TCTTATCCTG GCTCCCAGAC AATTCCGATG CAGAATTGGA GGAAGTCTCC 1321 GTGGAGAATG GTTCCGTCTC TCCCCCACCT TTTAAACAGT CTCCGAGAAT CCGACGCAAG 1381 GGTTGGCAAG CCCACCAACG ACCTGGGACC CGTGCAGAGG GTGAGAGTGA CTCCCAGGAT 1441 ATGGGAGATG CACACAAGTC ACCCAATATG GGACCAAACC CTGGAATGGA TGGTGACTGT 1501 GTATATGAAA ACTTGGCCTT CCAAAAGGAA GAAGACTTGG AAAAGAAGAG AGAGGCCTCT 1561 GAGTCTACAG GGACGAACTC CTCAGCAGCA CACAACGAAG AGTTGTCCAA GGCCCTGAAA 1621 GGCGAGGGTG GCACGGACAG CGACCATATG AGGCACGAAG CCTCCTTGGC AATCCGCTCC 1681 CCCTGCCCTG GGCTGGAGGA GGACATGGAA GCCTACGTGC TGCGGCCAGC GCTCCCGGGC 1741 ACCATGATGC AGTGCTACCT CACCCGTGAC AAGCACGGCG TGGACAAGGG CTTGTTCCCC 1801 CTCTACTACC TCTACCTGGA GACCTCTGAC AGCCTGCAGC GCTTCCTCCT GGCTGGGCGA 1861 AAGAGAAGAA GGAGCAAAAC TTCTAATTAC CTCATCTCCC TGGATCCTAC ACACCTATCT 1921 CGGGACGGGG ACAATTTCGT GGGCAAAGTC AGATCCAATG TCTTCAGCAC CAAGTTCACC 1981 ATCTTTGACA ATGGGGTGAA TCCTGACCGG GAGCATTTAA CCAGGAATAC TGCCCGGATC 2041 AGACAGGAGC TGGGGGCTGT GTGTTATGAG CCCAACGTCT TAGGATACCT GGGGCCTCGG 2101 AAAATGACTG TGATTCTCCC AGGAACCAAC AGCCAGAACC AGCGAATCAA TGTCCAGCCA 2161 CTAAATGAAC AGGAGTCGCT ACTGAGTCGT TACCAACGTG GGGACAAACA AGGGTTGCTT 2221 TTGTTGCACA ACAAAACCCC GTCGTGGGAC AAGGAGAACG GTGTCTACAC GCTCAATTTC 2281 CATGGTCGAG TCACTCGGGC TTCGGTGAAG AACTTCCAAA TCGTGGATCC CAAACACCAA 2341 GAACATCTGG TGCTCCAGTT CGGCCGAGTG GGCCCAGACA CATTCACCAT GGACTTCTGC 2401 TTTCCATTTA GCCCGCTCCA GGCCTTCAGC ATCTGCTTGT CCAGTTTCAA TtagGGTACC 2461 GCGGCCGCGG GGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 2521 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA 2581 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 2641 TTCAGGGGGA GGTGTGGGAG GTTTTTTCGG ATCCTCTAGA GTCGATCTGC AGGCATGCTA 2701 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 2761 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 2821 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 2881 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 2941 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 3001 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 3061 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 3121 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 3181 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 3241 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 3301 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 3361 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 3421 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 3481 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 3541 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 3601 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT 3661 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 3721 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 3781 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 3841 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 3901 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 3961 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 4021 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 4081 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 4141 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 4201 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 4261 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 4321 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 4381 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 4441 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 4501 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 4561 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 4621 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 4681 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 4741 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 4801 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 4861 TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA 4921 TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 4981 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 5041 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 5101 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 5161 AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG 5221 TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA 5281 GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.