pLV-PGK-MCS plasmid

pLV-PGK-MCS plasmid

PVT88302

pLV-PGK-MCS plasmid Information

Plasmid name: pLV-PGK-MCS
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: PGK
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: WPRE-R
Copy number: High
Induction:

pLV-PGK-MCS plasmid Description

pLV-PGK-MCS It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV-PGK-MCS plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt 2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca 2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat 2221 cgaattgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc 2281 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg 2341 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc 2401 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag 2461 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag 2521 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg 2581 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa 2641 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct 2701 cctcttcctc atctccgggc ctttcgaatt ctcacgcgtG CTAGCGTTAA GCTTGGTACC 2761 GAGCTCGGAT CCgcggccgc aactcccacc tgcaacatgc gtgactgact gaggccgcga 2821 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct 2881 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 2941 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 3001 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 3061 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 3121 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 3181 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 3241 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 3301 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 3361 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 3421 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg 3481 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta 3541 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 3601 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 3661 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 3721 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 3781 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct 3841 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa 3901 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc 3961 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt 4021 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta 4081 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat 4141 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt 4201 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca 4261 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct 4321 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg 4381 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 4441 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 4501 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 4561 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 4621 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 4681 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 4741 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 4801 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 4861 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 4921 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 4981 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 5041 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 5101 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 5161 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 5221 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 5281 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 5341 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 5401 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 5461 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 5521 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 5581 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 5641 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 5701 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 5761 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 5821 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 5881 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 5941 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6001 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 6061 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 6121 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 6181 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 6241 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 6301 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 6361 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 6421 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 6481 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 6541 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa 6601 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 6661 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 6721 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 6781 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 6841 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 6901 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga 6961 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg 7021 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt 7081 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa 7141 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga 7201 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc 7261 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga 7321 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt 7381 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta 7441 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga 7501 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt 7561 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt 7621 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca 7681 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct 7741 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg 7801 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca 7861 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta 7921 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt 7981 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta 8041 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa 8101 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag 8161 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt 8221 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta 8281 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt 8341 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac 8401 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat 8461 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct 8521 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg 8581 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc 8641 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 8701 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 8761 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 8821 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc 8881 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga 8941 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt 9001 agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.