pLV3-CMV-3×FLAG-SLC6A6-EF1a-EG

pLV3-CMV-3×FLAG-SLC6A6-EF1a-EGFP-Puro plasmid

PVT93970

pLV3-CMV-3×FLAG-SLC6A6-EF1a-EGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-3×FLAG-SLC6A6(human)-EF1a-EGFP-Puro
Alias: NM_003043.6;HTRDC; TAUT;
Gene lenght: 1863bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×FLAG-SLC6A6-EF1a-EGFP-Puro plasmid Description

pLV3-CMV-3×FLAG-SLC6A6-EF1a-EGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-3×FLAG-SLC6A6-EF1a-EGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg actacaaaga ccatgacggt 2341 gattataaag atcatgacat cgactacaag gatgacgatg acaagggatc catggccacc 2401 aaggagaagc tgcagtgtct gaaagatttc cacaaggaca tcctgaagcc ctcaccaggg 2461 aagagcccag gcacgcggcc tgaggacgag gctgagggaa aacctccgca gagggagaag 2521 tggtctagca agatcgactt tgtgctctct gtggctggcg gcttcgtggg cttgggcaac 2581 gtctggcgct tcccgtacct ctgctacaag aatggtggag gtgcgtttct cataccgtat 2641 tttattttcc tgtttgggag cggcctgcct gtgtttttct tggagatcat cataggccag 2701 tacacctctg aagggggcat cacctgctgg gaaaagatct gccccttgtt ctctggtatc 2761 ggctatgcct ccgttgtaat tgtgtccctc ctgaatgtct actacatcgt catcctggcc 2821 tgggccacat actacctgtt ccagtccttc cagaaggagc tgccctgggc acactgcaac 2881 cacagctgga acacacctca ctgcatggag gacaccatgc gcaagaacaa gagtgtctgg 2941 atcaccatca gctccaccaa cttcacctcc cctgtcatcg agttctggga gcgcaacgtg 3001 ctgagcttgt cccctggaat cgaccaccca ggctctctga aatgggacct cgctctctgc 3061 cttcttttag tctggctagt gtgtttcttc tgcatctgga agggcgtcag gtccactggg 3121 aaggtcgtct acttcacagc cacttttcca ttcgccatgc tcctggtgct gctggtccga 3181 gggctgacgc tgccgggcgc gggcgcaggc atcaagttct atctgtatcc tgacatcacc 3241 cgccttgagg acccacaggt gtggattgac gctgggactc agatattctt ctcttatgcc 3301 atctgcctgg gggctatgac ctcgctgggg agctacaaca agtacaagta taactcgtac 3361 agggactgta tgctgctggg atgcctgaac agtggtacca gttttgtgtc tggcttcgca 3421 attttttcca tcctgggctt catggcacaa gagcaagggg tggacattgc tgatgtggct 3481 gagtcaggtc ctggcctggc cttcattgcc tacccaaaag ctgtgacaat gatgccgctg 3541 cccacatttt ggtccattct tttttttatt atgcttctct tgcttggact ggatagccag 3601 tttgttgaag ttgaaggaca gatcacatcc ttggttgatc tttacccatc cttcctaagg 3661 aagggttatc gtcgggaaat cttcatcgcc ttcgtgtgta gcatcagcta cctgctgggg 3721 ctgacgatgg tgacggaggg tggcatgtat gtgtttcagc tctttgacta ctatgcagct 3781 agcggtgtat gccttttgtg ggttgcattc tttgaatgtt ttgttattgc ctggatatat 3841 ggaggtgata acctttatga tggtattgag gacatgattg gctatcggcc cgggccctgg 3901 atgaagtaca gctgggctgt gatcactcca gttctctgtg ttggatgttt catcttctcg 3961 ctcgtcaagt acgtacccct gacctacaac aaaacatacg tgtaccccaa ctgggccatt 4021 gggctgggct ggagcctggc cctttcctcc atgctctgcg ttcccttggt catcgtcatc 4081 cgcctctgcc agactgaggg gccgttcctt gtgagagtca agtacctgct gaccccaagg 4141 gaacccaacc gctgggctgt ggagcgcgag ggagccacac cttacaactc tcgcaccgtc 4201 atgaacggcg ctctcgtgaa accgacccac atcattgtgg agaccatgat gtgagcggcc 4261 gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 4321 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 4381 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 4441 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 4501 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 4561 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 4621 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 4681 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 4741 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 4801 ggcgcctacg ctagacgcca ccatgagcaa gggcgaggag ctgttcaccg gggtggtgcc 4861 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 4921 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 4981 gcccgtgccc tggcccaccc tcgtgaccac cttcagctac ggcgtgcagt gcttcagccg 5041 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 5101 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 5161 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 5221 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 5281 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 5341 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 5401 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga 5461 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcacggcat 5521 ggacgagctg tacaagagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc 5581 cggacccggc tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt 5641 ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 5701 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 5761 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 5821 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 5881 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 5941 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 6001 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 6061 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 6121 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 6181 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 6241 ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg 6301 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 6361 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 6421 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 6481 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 6541 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 6601 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaATCATC 6661 GTCCTTTCCT tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 6721 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 6781 gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggGccgc 6841 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 6901 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaGa taagatctgc 6961 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 7021 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 7081 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 7141 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 7201 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat 7261 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 7321 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctaTCC CGCCCCTAAC 7381 TCCGCCCAtc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 7441 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 7501 gtgaggaggc ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat 7561 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 7621 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 7681 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 7741 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 7801 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 7861 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 7921 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 7981 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 8041 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 8101 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 8161 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 8221 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 8281 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 8341 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 8401 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 8461 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 8521 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 8581 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 8641 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 8701 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 8761 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 8821 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 8881 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 8941 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 9001 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 9061 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 9121 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 9181 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 9241 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 9301 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 9361 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 9421 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 9481 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 9541 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 9601 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 9661 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 9721 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg 9781 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag 9841 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg 9901 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc 9961 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt 10021 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 10081 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt 10141 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.