pCMV-EZH2-FLAG-Neo plasmid

pCMV-EZH2-FLAG-Neo plasmid

PVT87636

pCMV-EZH2-FLAG-Neo plasmid Information

Plasmid name: pCMV-EZH2(human)-FLAG-Neo
Alias: NM_004456.5;ENX-1; ENX1; EZH2b; KMT6; KMT6A; WVS; WVS2
Gene lenght: 2253bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS2-FLAG-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-EZH2-FLAG-Neo plasmid Description

pCMV-EZH2-FLAG-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and CMV promoter that work together to efficiently express the human EZH2 gene, promoting the C-terminal fusion expression of the EZH2 gene with a FLAG tag, facilitating detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The plasmid carries a Neo/G418 resistance gene for selecting host cells expressing the target gene.

pCMV-EZH2-FLAG-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcGCCAcc atgggccaga ctgggaagaa 661 atctgagaag ggaccagttt gttggcggaa gcgtgtaaaa tcagagtaca tgcgactgag 721 acagctcaag aggttcagac gagctgatga agtaaagagt atgtttagtt ccaatcgtca 781 gaaaattttg gaaagaacgg aaatcttaaa ccaagaatgg aaacagcgaa ggatacagcc 841 tgtgcacatc ctgacttctg tgagctcatt gcgcgggact agggagtgtt cggtgaccag 901 tgacttggat tttccaacac aagtcatccc attaaagact ctgaatgcag ttgcttcagt 961 acccataatg tattcttggt ctcccctaca gcagaatttt atggtggaag atgaaactgt 1021 tttacataac attccttata tgggagatga agttttagat caggatggta ctttcattga 1081 agaactaata aaaaattatg atgggaaagt acacggggat agagaatgtg ggtttataaa 1141 tgatgaaatt tttgtggagt tggtgaatgc ccttggtcaa tataatgatg atgacgatga 1201 tgatgatgga gacgatcctg aagaaagaga agaaaagcag aaagatctgg aggatcaccg 1261 agatgataaa gaaagccgcc cacctcggaa atttccttct gataaaattt ttgaagccat 1321 ttcctcaatg tttccagata agggcacagc agaagaacta aaggaaaaat ataaagaact 1381 caccgaacag cagctcccag gcgcacttcc tcctgaatgt acccccaaca tagatggacc 1441 aaatgctaaa tctgttcaga gagagcaaag cttacactcc tttcatacgc ttttctgtag 1501 gcgatgtttt aaatatgact gcttcctaca tcgtaagtgc aattattctt ttcatgcaac 1561 acccaacact tataagcgga agaacacaga aacagctcta gacaacaaac cttgtggacc 1621 acagtgttac cagcatttgg agggagcaaa ggagtttgct gctgctctca ccgctgagcg 1681 gataaagacc ccaccaaaac gtccaggagg ccgcagaaga ggacggcttc ccaataacag 1741 tagcaggccc agcaccccca ccattaatgt gctggaatca aaggatacag acagtgatag 1801 ggaagcaggg actgaaacgg ggggagagaa caatgataaa gaagaagaag agaagaaaga 1861 tgaaacttcg agctcctctg aagcaaattc tcggtgtcaa acaccaataa agatgaagcc 1921 aaatattgaa cctcctgaga atgtggagtg gagtggtgct gaagcctcaa tgtttagagt 1981 cctcattggc acttactatg acaatttctg tgccattgct aggttaattg ggaccaaaac 2041 atgtagacag gtgtatgagt ttagagtcaa agaatctagc atcatagctc cagctcccgc 2101 tgaggatgtg gatactcctc caaggaaaaa gaagaggaaa caccggttgt gggctgcaca 2161 ctgcagaaag atacagctga aaaaggacgg ctcctctaac catgtttaca actatcaacc 2221 ctgtgatcat ccacggcagc cttgtgacag ttcgtgccct tgtgtgatag cacaaaattt 2281 ttgtgaaaag ttttgtcaat gtagttcaga gtgtcaaaac cgctttccgg gatgccgctg 2341 caaagcacag tgcaacacca agcagtgccc gtgctacctg gctgtccgag agtgtgaccc 2401 tgacctctgt cttacttgtg gagccgctga ccattgggac agtaaaaatg tgtcctgcaa 2461 gaactgcagt attcagcggg gctccaaaaa gcatctattg ctggcaccat ctgacgtggc 2521 aggctggggg atttttatca aagatcctgt gcagaaaaat gaattcatct cagaatactg 2581 tggagagatt atttctcaag atgaagctga cagaagaggg aaagtgtatg ataaatacat 2641 gtgcagcttt ctgttcaact tgaacaatga ttttgtggtg gatgcaaccc gcaagggtaa 2701 caaaattcgt tttgcaaatc attcggtaaa tccaaactgc tatgcaaaag ttatgatggt 2761 taacggtgat cacaggatag gtatttttgc caagagagcc atccagactg gcgaagagct 2821 gttttttgat tacagataca gccaggctga tgccctgaag tatgtcggca tcgaaagaga 2881 aatggaaatc cctggatccG ATTACAAGGA CGACGATGAC AAGtagcggc cgcgactcta 2941 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 3001 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 3061 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 3121 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg 3181 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 3241 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 3301 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 3361 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 3421 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 3481 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 3541 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 3601 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg 3661 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 3721 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca 3781 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 3841 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 3901 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 3961 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 4021 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 4081 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat 4141 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 4201 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 4261 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 4321 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 4381 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 4441 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 4501 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 4561 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 4621 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 4681 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 4741 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 4801 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 4861 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 4921 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 4981 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 5041 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 5101 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc 5161 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc 5221 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag 5281 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc 5341 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg 5401 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 5461 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 5521 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 5581 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 5641 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 5701 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 5761 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 5821 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 5881 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 5941 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 6001 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 6061 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 6121 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 6181 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 6241 ccgtattacc gccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.