pLV2-CMV-SLC16A1-3×FLAG-Puro p

pLV2-CMV-SLC16A1-3×FLAG-Puro plasmid

PVT87585

pLV2-CMV-SLC16A1-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-SLC16A1(human)-3×FLAG-Puro
Alias: NM_003051.4;HHF7; MCT; MCT1; MCT1D
Gene lenght: 1500bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLVX-3×FLAG-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-SLC16A1-3×FLAG-Puro plasmid Description

pLV2-CMV-SLC16A1-3×FLAG-Puro This is a plasmid suitable for overexpression in mammalian cells. The combination of the CMV enhancer and CMV promoter initiates transcription of the human SLC16A1 gene, promoting the C-terminal fusion expression of the SLC16A1 gene with a 3×FLAG tag, allowing for detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating stable integration of the gene into host cells. The plasmid also carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV2-CMV-SLC16A1-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgccacc agcagttgga ggtccagttg gatacacccc 2881 cccagatgga ggctggggct gggcagtggt aattggagct ttcatttcca tcggcttctc 2941 ttatgcattt cccaaatcaa ttactgtctt cttcaaagag attgaaggta tattccatgc 3001 caccaccagc gaagtgtcat ggatatcctc cataatgttg gctgtcatgt atggtggagg 3061 tcctatcagc agtatcctgg tgaataaata tggaagtcgt atagtcatga ttgttggtgg 3121 ctgcttgtca ggctgtggct tgattgcagc ttctttctgt aacaccgtac agcaactata 3181 cgtctgtatt ggagtcattg gaggtcttgg gcttgccttc aacttgaatc cagctctgac 3241 catgattggc aagtatttct acaagaggcg accattggcc aacggactgg ccatggcagg 3301 cagccctgtg ttcctctgta ctctggcccc cctcaatcag gttttcttcg gtatctttgg 3361 atggagagga agctttctaa ttcttggggg cttgctacta aactgctgtg ttgctggagc 3421 cctcatgcga ccaatcgggc ccaagccaac caaggcaggg aaagataagt ctaaagcatc 3481 ccttgagaaa gctggaaaat ctggtgtgaa aaaagatctg catgatgcaa atacagatct 3541 tattggaaga caccctaaac aagagaaacg atcagtcttc caaacaatta atcagttcct 3601 ggacttaacc ctattcaccc acagaggctt tttgctatac ctctctggaa atgtgatcat 3661 gttttttgga ctctttgcac ctttggtgtt tcttagtagt tatgggaaga gtcagcatta 3721 ttctagtgag aagtctgcct tccttctttc cattctggct tttgttgaca tggtagcccg 3781 accatctatg ggacttgtag ccaacacaaa gccaataaga cctcgaattc agtatttctt 3841 tgcggcttcc gttgttgcaa atggagtgtg tcatatgcta gcacctttat ccactaccta 3901 tgttggattc tgtgtctatg cgggattctt tggatttgcc ttcgggtggc tcagctccgt 3961 attgtttgaa acattgatgg accttgttgg accccagagg ttctccagcg ctgtgggatt 4021 ggtgaccatt gtggaatgct gtcctgtcct cctggggcca ccacttttag gtcggctcaa 4081 tgacatgtat ggagactaca aatacacata ctgggcatgt ggcgtcgtcc taattatttc 4141 aggtatctat ctcttcattg gcatgggcat caattatcga cttttggcaa aagaacagaa 4201 agcaaacgag cagaaaaagg aaagtaaaga ggaagagacc agtatagatg ttgctgggaa 4261 gccaaatgaa gttaccaaag cagcagaatc tccggaccag aaagacacag atggagggcc 4321 caaggaggag gaaagtccag tcggatccGA CTACAAAGAC CATGACGGTG ATTATAAAGA 4381 TCATGACATC GACTACAAGG ATGACGATGA CAAGtagtct agataattct accgggtagg 4441 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 4501 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 4561 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 4621 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 4681 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 4741 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 4801 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 4861 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 4921 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc 4981 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 5041 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5101 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5161 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5221 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5281 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 5341 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5401 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5461 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5521 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga 5581 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5641 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5701 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5761 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5821 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5881 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5941 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 6001 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 6061 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 6121 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 6181 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 6241 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 6301 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 6361 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 6421 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 6481 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 6541 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 6601 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 6661 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 6721 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 6781 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 6841 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6901 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6961 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 7021 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 7081 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 7141 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 7201 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 7261 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 7321 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 7381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 7441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 7501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 7561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 7621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 7681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 7741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 7801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 7861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 7981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 8041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 8101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 8161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 8221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 8281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 8341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 8401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 8461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 8521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 8581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 8641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 8701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 8761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 8821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 8881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 8941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 9001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 9061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 9121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 9181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 9241 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac 9301 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 9361 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 9421 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 9481 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 9541 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt 9601 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.