pCMV-HECW1-Neo plasmid

pCMV-HECW1-Neo plasmid

PVT91578

pCMV-HECW1-Neo plasmid Information

Plasmid name: pCMV-HECW1(human-gc-opt)-Neo
Alias: NM_015052.5;NEDL1;
Gene lenght: 4818bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-HECW1-Neo plasmid Description

pCMV-HECW1-Neo

pCMV-HECW1-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg ctgctgcacc tgtgtagtgt gaagaatctg taccagaaca ggtttttagg 961 cctggccgcc atggcgtctc cttctagaaa ctcccagagc cgacgccggt gcaaggagcc 1021 gctccgatac agctacaacc ccgaccagtt ccacaacatg gacctcaggg gcggccccca 1081 cgatggcgtc accattcccc gctccaccag cgacactgac ctggtcacct cggacagccg 1141 ctccacgctc atggtcagca gctcctacta ttccatcggg cactctcagg acctggtcat 1201 ccactgggac ataaaggagg aagtggacgc tggggactgg attggcatgt acctcattga 1261 tgaggtcttg tccgaaaact ttctggacta taaaaaccgt ggagtcaatg gttctcatcg 1321 gggccagatc atctggaaga tcgatgccag ctcgtacttt gtggaacctg aaactaagat 1381 ctgcttcaaa tactaccatg gagtgagtgg ggccctgcga gcaaccaccc ccagtgtcac 1441 ggtcaaaaac tcggcagctc ctatttttaa aagcattggt gctgatgaga ccgtccaagg 1501 acaaggaagt cggaggctga tcagcttctc tctctcagat ttccaagcca tggggttgaa 1561 gaaagggatg tttttcaacc cagaccctta tctgaagatt tccattcagc ctgggaaaca 1621 cagcatcttc cccgccctcc ctcaccatgg acaggagagg agatccaaga tcataggcaa 1681 caccgtgaac cccatctggc aggccgagca attcagtttt gtgtccttgc ccactgacgt 1741 gctggaaatt gaggtgaagg acaagtttgc caagagccgc cccatcatca agcgcttctt 1801 gggaaagctg tcgatgcccg ttcaaagact cctggagaga cacgccatag gggatagggt 1861 ggtcagctac acacttggcc gcaggcttcc aacagatcat gtgagtggac agctgcaatt 1921 ccgatttgag atcacttcct ccatccaccc agatgatgag gagatttccc tgagtaccga 1981 gcctgagtca gcccaaattc aggacagccc catgaacaac ctgatggaaa gcggcagtgg 2041 ggaacctcgg tctgaggcac cagagtcctc tgagagctgg aagccagagc agctgggtga 2101 gggcagtgtc cccgatggtc cagggaacca aagcatagag ctttccagac cagctgagga 2161 agcagcagtc atcacggagg caggagacca gggcatggtc tctgtgggac ctgaaggggc 2221 tggggagctc ctggcccagg tgcaaaagga catccagcct gcccccagtg cagaagagct 2281 ggccgagcag ctggacctgg gtgaggaggc atcagcactg ctgctggaag acggtgaagc 2341 cccagccagc accaaggagg agcccttgga ggaggaagca acgacccaga gccgggctgg 2401 aagggaagaa gaggagaagg agcaggagga ggagggagat gtgtctaccc tggagcaggg 2461 agagggcagg ctgcagctgc gggcctcggt gaagagaaaa agcaggccct gctccttgcc 2521 tgtgtccgag ctggagacgg tgatcgcgtc agcctgcggg gaccccgaga ccccgcggac 2581 acactacatc cgcatccaca ccctgctgca cagcatgccc tccgcccagg gcggcagcgc 2641 ggcagaggag gaggacggcg cggaggagga gtccaccctc aaggactcct cggagaagga 2701 tgggctcagc gaggtggaca cggtggccgc tgacccgtct gccctggaag agGATAGAGA 2761 GGAGCCCGAA GGAGCAACTC CTGGTACTGC TCATCCTGGG CACTCTGGCG GACACTTTCC 2821 TTCTCTGGCT AACGGGGCTG CCCAGGATGG CGATACCCAC CCTTCCACCG GAAGCGAGTC 2881 TGATTCATCA CCTAGACAGG GCGGCGATCA CAGTTGCGAA GGCtgtgacg cgtcctgctg 2941 cagcccctcg tgctacagct cctcgtgcta cagcacgtcc tgctacagca gctcgtgcta 3001 cagcgcctcg tgctacagcc cctcctgcta caacggcaac aggttcgcca gccacacgcg 3061 cttctcctcc gtggacagcg ccaagatctc cgagagcacg gtcttctcct cgcaagacga 3121 cgaggaggag gagaacagcg cgttcgagtc ggtacccgac tccatgcaga gccctgagct 3181 ggacccggag tccacgaacg gcgctgggcc gtggcaagac gagctggccg cccctagcgg 3241 gcacgtggaa agaagcccgg aaggtctgga atcccccgtg gcaggtccaa gcaatcggag 3301 agaaggtgaa tgtcctatac tccataattc ccagccagta agccagcttc cttccctgag 3361 gcctgaacat catcactacc caacaatcga tgagcctctt ccaccaaact gggaagctcg 3421 aattgacagc cacgggcggg tcttttatgt ggaccacgtg aaccgcacaa ccacctggca 3481 gcgtccgacg gcagcagcca ccccggatgg catgcggaga tcggggtcca tccagcagat 3541 ggagcaactc aacaggcggt atcaaaacat tcagcgaacc attgcaacag agaggtccga 3601 agaagattct ggcagccaaa gctgcgagca agccccagca ggaggaggcg gaggtggagg 3661 gagtgactca gaagccgaat cttcccagtc cagcttagat ctaaggagag aagggtcact 3721 ttctccagtg aactcacaaa aaatcacctt gctgctgcag tccccagcgg tcaagttcat 3781 caccaacccc gagttcttca ctgtgctaca tgccaattat agtgcctacc gagtcttcac 3841 cagtagcacc tgcttaaagc acatgattct gaaagtccga cgggatgctc gcaattttga 3901 acgctaccag cacaaccggg acttggtgaa tttcatcaac atgttcgcag acactcggct 3961 ggaactgccc cggggctggg agatcaaaac ggaccagcag ggaaagtctt ttttcgtgga 4021 ccacaacagt cgagctacca ctttcattga cccccgaatc cctcttcaga acggtcgtct 4081 tcccaatcat ctaactcacc gacagcacct ccagaggctc cgaagttaca gcgctggaga 4141 ggcctcagaa gtttctagaa acagaggagc ctctttactg gccaggccag gacacagctt 4201 agtagctgct attcgaagcc aacatcaaca tgagtcattg ccactggcat ataatgacaa 4261 gattgtggca tttcttcgcc agccaaacat ttttgaaatg ctgcaagagc gtcagccaag 4321 cttagcaaga aaccacacac tcagggagaa aatccattac attcggactg agggtaatca 4381 cgggcttgag aagttgtcct gtgatgcgga tctggtcatt ttgctgagtc tctttgaaga 4441 agagattatg tcctacgtcc ccctgcaggc tgccttccac cctgggtata gcttctctcc 4501 ccgatgttca ccctgttctt cacctcagaa ctccccaggt ttacagagag ccagtgcaag 4561 agccccttcc ccctaccgaa gagactttga ggccaagctc cgcaatttct acagaaaact 4621 ggaagccaaa ggatttggtc agggtccggg gaaaattaag ctcattattc gccgggatca 4681 tttgttggag ggaaccttca atcaggtgat ggcctattcg cggaaagagc tccagcgaaa 4741 caagctctac gtcacctttg ttggagagga gggcctggac tacagtggcc cctcgcggga 4801 gttcttcttc cttctgtctc aggagctctt caacccttac tatggactct ttgagtactc 4861 ggcaaatgat acttacacgg tgcagatcag ccccatgtcc gcatttgtag aaaaccatct 4921 tgagtggttc aggtttagcg gtcgcatcct gggtctggct ctgatccatc agtaccttct 4981 tgacgctttc ttcacgaggc ccttctacaa ggcactcctg agactgccct gtgatttgag 5041 tgacctggaa tatttggatg aggaattcca ccagagtttg cagtggatga aggacaacaa 5101 catcacagac atcttagacc tcactttcac tgttaatgaa gaggtttttg gacaggtcac 5161 ggaaagggag ttgaagtctg gaggagccaa cacacaggtg acggagaaaa acaagaagga 5221 gtacatcgag cgcatggtga agtggcgggt ggagcgcggc gtggtacagc agaccgaggc 5281 gctggtgcgc ggcttctacg aggttgtaga ctcgaggctg gtgtccgtgt ttgatgccag 5341 ggagctggag ctggtgatag ctggcaccgc ggaaatcgac ctaaatgact ggcggaataa 5401 cactgagtac cggggaggtt accacgatgg gcatcttgtg atccgctggt tctgggctgc 5461 ggtggagcgc ttcaataatg agcagaggct gagattactg cagtttgtca cgggaacatc 5521 cagcgtgccc tacgaaggct tcgcagccct ccgtgggagc aatgggcttc ggcgcttctg 5581 catagagaaa tgggggaaaa ttacttctct ccccagggca cacacatgct tcaaccgact 5641 ggatcttcca ccgtatccct cgtactccat gttgtatgaa aagctgttaa cagcagtaga 5701 ggaaaccagc acctttggac ttgagtgaCT CGAGTCTAGA GGGCCCGTTT AAACCCGCTG 5761 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 5821 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 5881 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 5941 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC 6001 TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC 6061 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT 6121 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG 6181 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA 6241 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 6301 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG 6361 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 6421 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG 6481 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 6541 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 6601 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 6661 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 6721 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA 6781 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 6841 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 6901 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 6961 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 7021 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 7081 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 7141 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 7201 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 7261 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 7321 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 7381 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 7441 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 7501 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 7561 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 7621 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 7681 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 7741 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 7801 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 7861 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 7921 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 7981 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 8041 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 8101 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 8161 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 8221 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 8281 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 8341 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 8401 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 8461 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 8521 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 8581 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 8641 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 8701 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 8761 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 8821 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 8881 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 8941 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 9001 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 9061 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 9121 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 9181 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 9241 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 9301 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 9361 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 9421 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 9481 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 9541 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 9601 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 9661 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 9721 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 9781 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 9841 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 9901 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 9961 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 10021 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 10081 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 10141 GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT 10201 ATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.