pLV3-CMV-AARS1-3×FLAG-Puro pla

pLV3-CMV-AARS1-3×FLAG-Puro plasmid

PVT87461

pLV3-CMV-AARS1-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-AARS1(human)-3×FLAG-Puro
Alias: NM_001605.3;AARS; CMT2N; DEE29; EIEE29; HDLS2; TTD8
Gene lenght: 2904bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-AARS1-3×FLAG-Puro plasmid Description

pLV3-CMV-AARS1-3×FLAG-Puro This plasmid is suitable for overexpression in mammalian cells. The CMV strong promoter carried by the plasmid can initiate the transcription of the human AARS1 gene, promoting the C-terminal fusion expression of the AARS1 gene with a 3×FLAG tag, facilitating detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid contains HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The plasmid carries a Puro resistance gene for screening host cells expressing the target gene.

pLV3-CMV-AARS1-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggactct actctaacag caagtgaaat ccggcagcga 2341 tttatagatt tcttcaagag gaacgagcat acgtatgttc actcgtctgc caccatccca 2401 ttggatgacc ccactttgct ctttgccaat gcaggcatga accagtttaa acccattttc 2461 ctgaacacaa ttgacccatc tcaccccatg gcaaagctga gcagagctgc caatacccag 2521 aagtgcatcc gggctggggg caaacataat gacctggacg atgtgggcaa ggatgtctat 2581 catcacacct tcttcgagat gctgggctct tggtcttttg gagattactt taaggaattg 2641 gcatgtaaga tggctctgga actcctcacc caagagtttg gcattcccat tgaaagactt 2701 tatgttactt actttggcgg ggatgaagca gctggcttag aagcagatct ggaatgcaaa 2761 cagatctggc aaaatttggg gctggatgac accaaaatcc tcccaggcaa catgaaggat 2821 aacttctggg agatgggtga cacgggcccc tgtggtcctt gcagtgagat ccactacgac 2881 cggattggtg gtcgggacgc cgcacatctt gtcaaccagg acgaccctaa tgtgctggag 2941 atctggaacc ttgtgttcat ccagtataac agggaagctg atggcattct gaaacctctt 3001 cccaagaaaa gcattgacac agggatgggc ctggaacgac tggtatctgt gctgcagaat 3061 aagatgtcca actatgacac tgaccttttt gtcccttact ttgaagccat tcagaagggc 3121 acaggtgccc gaccatacac tgggaaagtt ggtgctgagg atgccgatgg gattgacatg 3181 gcctaccggg tgctggctga ccacgctcgg accatcactg tggcactggc tgatggtggc 3241 cggcctgaca acacagggcg tggatatgtg ttgagacgga ttctccgccg agctgtccga 3301 tacgcccatg aaaagctcaa tgccagcagg ggcttctttg ctacgttagt ggatgttgtc 3361 gtccagtccc tgggagatgc atttcctgag ctgaagaagg acccagacat ggtgaaggac 3421 atcattaatg aagaagaggt gcagtttctc aagactctca gcagagggcg tcgcatcctg 3481 gacaggaaaa ttcagagcct gggagacagc aagaccattc ccggagacac tgcttggctc 3541 ctctatgaca cctatgggtt tccagtggat ctgactggac tgattgctga agagaagggc 3601 ctggtggtag acatggatgg ctttgaagag gagaggaaac tggcccagct gaaatcacag 3661 ggcaagggag ctggtgggga agacctcatt atgctggaca tttacgctat cgaagagctc 3721 cgggcacggg gtctggaggt cacagatgat tccccaaagt acaattacca tttggactcc 3781 agtggtagct atgtatttga gaacacagtg gctacggtga tggctctgcg cagggagaag 3841 atgttcgtgg aagaggtgtc cacaggccag gagtgtggag tggtgctgga caagacctgt 3901 ttctatgctg agcaaggagg ccagatctat gacgaaggct acctggtgaa ggtggatgac 3961 agcagtgaag ataaaacaga gtttacagtg aagaatgctc aggtccgagg agggtatgtg 4021 ctacacattg gaaccatcta cggtgacctg aaagtggggg atcaggtctg gctgtttatt 4081 gatgagcccc gacgaagacc catcatgagc aaccacacag ctacgcacat tctgaacttc 4141 gccctgcgct cagtgcttgg ggaagctgac cagaaaggct cattggttgc tcctgaccgc 4201 ctcagatttg actttactgc caagggagcc atgtccaccc aacagatcaa gaaggctgaa 4261 gagattgcta atgagatgat tgaggcagcc aaggccgtct atacccagga ttgccccctg 4321 gcagcagcga aagccatcca gggcctacgg gctgtgtttg atgagaccta tcctgaccct 4381 gtgcgagtcg tctccattgg ggtcccggtg tccgagttgc tggatgaccc ctctgggcct 4441 gctggctccc tgacttctgt tgagttctgt gggggaacgc acctgcggaa ctcgagtcat 4501 gcaggagctt ttgtgatcgt gacggaagaa gccattgcca agggtatccg gaggattgtg 4561 gctgtcacag gtgccgaggc ccagaaggcc ctcaggaaag cagagagctt gaagaaatgt 4621 ctctctgtca tggaagccaa agtgaaggct cagactgctc caaacaagga tgtgcagagg 4681 gagatcgctg accttggaga ggccctggcc actgcagtca tcccccagtg gcagaaggat 4741 gaattgcggg agactctcaa atccctaaag aaggtcatgg atgacttgga ccgagccagc 4801 aaagccgatg tccagaaacg agtgttagag aagacgaagc agttcatcga cagcaacccc 4861 aaccagcctc ttgtcatcct ggagatggag agcggcgcct cagccaaggc cctgaatgaa 4921 gccttgaagc tcttcaagat gcactcccct cagacttctg ccatgctctt cacggtggac 4981 aatgaggctg gcaagatcac gtgcctgtgt caagttcccc agaatgcagc caatcggggc 5041 ttaaaagcca gcgagtgggt gcagcaggtg tcaggcttga tggacggtaa aggtggtggc 5101 aaggatgtgt ctgcacaggc cacaggcaag aacgttggct gcctgcagga ggcgctgcag 5161 ctggccactt ccttcgccca gctgcgcctc ggggatgtaa agaacGGATC CGACTACAAA 5221 GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG 5281 CGGCCGCGAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC 5341 ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG 5401 CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG 5461 GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC 5521 GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT 5581 ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC 5641 TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT 5701 CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG 5761 CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG 5821 TGACCGGCGC CTACGCTAGA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA 5881 CGACGTCCCC CGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG 5941 CCACACCGTC GACCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT 6001 CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC 6061 GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG 6121 CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC 6181 GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA 6241 CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC 6301 CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT 6361 CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC 6421 CCGCAAGCCC GGTGCCTGAG TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT 6481 GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC 6541 TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG 6601 GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC 6661 TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC 6721 CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC 6781 CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA 6841 ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC 6901 CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC 6961 GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG 7021 GGCCGCCTCC CCGCCTGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA 7081 GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAAATAAG 7141 ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT 7201 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG 7261 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT 7321 CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG TATTTATAAC 7381 TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT TATTGCAGCT TATAATGGTT 7441 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 7501 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGCTCTAG CTATCCCGCC 7561 CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA 7621 TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT 7681 TGGAGGCCTA GACTTTTGCA GAGACGGCCC AAATTCGTAA TCATGGTCAT AGCTGTTTCC 7741 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 7801 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 7861 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 7921 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 7981 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 8041 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 8101 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 8161 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 8221 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 8281 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 8341 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 8401 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 8461 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 8521 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG 8581 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 8641 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 8701 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 8761 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 8821 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 8881 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 8941 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 9001 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 9061 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 9121 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 9181 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 9241 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 9301 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 9361 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 9421 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 9481 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 9541 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 9601 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 9661 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 9721 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 9781 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 9841 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT 9901 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG 9961 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA 10021 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG 10081 GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG 10141 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG 10201 GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC 10261 GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG 10321 ACGTTGTAAA ACGACGGCCA GTGCCAAGCT G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.