pBiFC-ENO1-VC155 plasmid

pBiFC-ENO1-VC155 plasmid

PVT87330

pBiFC-ENO1-VC155 plasmid Information

Plasmid name: pBiFC-ENO1(human)-VC155
Alias: NM_001428.5;ENO1-IT1; ENO1L1; HEL-S-17; MPB1; NNE; PPH
Gene lenght: 1302bp
Host: Mammalian cells
Use(s): Bimolecular fluorescence
Fragment type: BIFC
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pBiFC-VC155
Forward primer: SV40 intron-F1
Reverse primer:
Copy number: High
Induction:

pBiFC-ENO1-VC155 plasmid Description

pBiFC-ENO1-VC155 This plasmid is suitable for detecting protein interactions in mammalian cells using a bimolecular fluorescence complementation assay. The plasmid contains a strong CMV promoter that can initiate the transcription of the human-derived ENO1 gene. The plasmid carries the VC155 reporter gene, which can bind to the N-terminal fragment VN173 of the yellow fluorescent protein (Venus), restoring fluorescent activity for detecting interactions between proteins.

pBiFC-ENO1-VC155 plasmid Sequence

1 ATGTACCCAT ACGATGTTCC AGATTACGCT CTTATGGCCA TGGAGGCCCG AATTatgtct 61 attctcaaga tccatgccag ggagatcttt gactctcgcg ggaatcccac tgttgaggtt 121 gatctcttca cctcaaaagg tctcttcaga gctgctgtgc ccagtggtgc ttcaactggt 181 atctatgagg ccctagagct ccgggacaat gataagactc gctatatggg gaagggtgtc 241 tcaaaggctg ttgagcacat caataaaact attgcgcctg ccctggttag caagaaactg 301 aacgtcacag aacaagagaa gattgacaaa ctgatgatcg agatggatgg aacagaaaat 361 aaatctaagt ttggtgcgaa cgccattctg ggggtgtccc ttgccgtctg caaagctggt 421 gccgttgaga agggggtccc cctgtaccgc cacatcgctg acttggctgg caactctgaa 481 gtcatcctgc cagtcccggc gttcaatgtc atcaatggcg gttctcatgc tggcaacaag 541 ctggccatgc aggagttcat gatcctccca gtcggtgcag caaacttcag ggaagccatg 601 cgcattggag cagaggttta ccacaacctg aagaatgtca tcaaggagaa atatgggaaa 661 gatgccacca atgtggggga tgaaggcggg tttgctccca acatcctgga gaataaagaa 721 ggcctggagc tgctgaagac tgctattggg aaagctggct acactgataa ggtggtcatc 781 ggcatggacg tagcggcctc cgagttcttc aggtctggga agtatgacct ggacttcaag 841 tctcccgatg accccagcag gtacatctcg cctgaccagc tggctgacct gtacaagtcc 901 ttcatcaagg actacccagt ggtgtctatc gaagatccct ttgaccagga tgactgggga 961 gcttggcaga agttcacagc cagtgcagga atccaggtag tgggggatga tctcacagtg 1021 accaacccaa agaggatcgc caaggccgtg aacgagaagt cctgcaactg cctcctgctc 1081 aaagtcaacc agattggctc cgtgaccgag tctcttcagg cgtgcaagct ggcccaggcc 1141 aatggttggg gcgtcatggt gtctcatcgt tcgggggaga ctgaagatac cttcatcgct 1201 gacctggttg tggggctgtg cactgggcag atcaagactg gtgccccttg ccgatctgag 1261 cgcttggcca agtacaacca gctcctcaga attgaagagg agctgggcag caaggctaag 1321 tttgccggca ggaacttcag aaaccccttg gccaagGGTA CCCGTCCGGC GTGCAAAATC 1381 CCGAACGACC TGAAACAGAA AGTCATGAAC CACGACAAGC AGAAGAACGG CATCAAGGCC 1441 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG 1501 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC 1561 CAGTCCAAAC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 1621 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAGC GGCCGCGGGG 1681 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA 1741 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT 1801 GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG 1861 TGTGGGAGGT TTTTTCGGAT CCTCTAGAGT CGATCTGCAG GCATGCTAGC TTGGCGTAAT 1921 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 1981 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 2041 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 2101 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 2161 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 2221 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 2281 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 2341 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 2401 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 2461 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 2521 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 2581 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 2641 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 2701 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 2761 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 2821 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA 2881 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 2941 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 3001 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 3061 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 3121 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 3181 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 3241 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 3301 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 3361 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 3421 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 3481 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 3541 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 3601 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 3661 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 3721 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 3781 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 3841 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 3901 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 3961 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 4021 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 4081 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT 4141 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 4201 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 4261 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 4321 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 4381 GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT 4441 CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC 4501 CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAGTTCGAG CTTGCATGCC 4561 TGCAGGTCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC 4621 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT 4681 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC 4741 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG 4801 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG 4861 CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT 4921 CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA 4981 ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA 5041 GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT 5101 GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC 5161 GGACTCTAGA GGATCCGGTA CTAGAGGAAC TGAAAAACCA GAAAGTTAAC TGGTAAGTTT 5221 AGTCTTTTTG TCTTTTATTT CAGGTCCCGG ATCCGGTGGT GGTGCAAATC AAAGAACTGC 5281 TCCTCAGTGG ATGTTGCCTT TACTTCTAGG CCTGTACGGA AGTGTTACTT CTGCTCTAAA 5341 AGCTGCGGAA TTGTACCCGC GGGCCCACC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.