pLV3-CMV-ILF3-3×Myc-EF1a-CopGF

pLV3-CMV-ILF3-3×Myc-EF1a-CopGFP-Puro plasmid

PVT86672

pLV3-CMV-ILF3-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ILF3(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_012218.4;CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4;MPP4110; NF-AT-90; NF110; NF110b; NF90; NF90a; NF90b;NF90c; NF90ctv; NFAR; NFAR-1; NFAR-2; NFAR110; NFAR2;NFAR90; TCP110; TCP80
Gene lenght: 2682bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×Myc-EF1a-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ILF3-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-ILF3-3×Myc-EF1a-CopGFP-Puro This plasmid is suitable for overexpression in mammalian cells. The CMV strong promoter can initiate the transcription of downstream genes, promoting the C-terminal fusion expression of a 3×Myc tag, which facilitates recognition and tracking of the Myc-tagged target protein by anti-Myc antibodies in experiments such as Western blotting, immunoprecipitation, or immunofluorescence, thereby studying the expression level and interactions of the target protein with other proteins. The plasmid carries a CopGFP green fluorescent protein driven by the EF1a promoter for detecting the expression and localization of the target gene. Additionally, the plasmid contains HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating stable integration of the gene into host cells. The plasmid carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV3-CMV-ILF3-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTTAACTTT 1801 TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG ACATAATAGC 1861 AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTATCGA 1921 TACTAGTATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC 1981 GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA 2041 TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG 2101 TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG 2161 CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC 2221 CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ATTCTAGAGC 2281 TAGCGAATTC GCCACCATGC GTCCAATGCG AATTTTTGTG AATGATGACC GCCATGTGAT 2341 GGCAAAGCAT TCTTCCGTTT ATCCAACACA AGAGGAGCTG GAGGCAGTCC AGAACATGGT 2401 GTCCCACACG GAGCGGGCGC TCAAAGCTGT GTCCGACTGG ATAGACGAGC AGGAAAAGGG 2461 TAGCAGCGAG CAGGCAGAGT CCGATAACAT GGATGTGCCC CCAGAGGACG ACAGTAAAGA 2521 AGGGGCTGGG GAACAGAAGA CGGAGCACAT GACCAGAACC CTGCGGGGAG TGATGCGGGT 2581 GGGCCTGGTG GCAAAGGGCC TCCTACTCAA GGGGGACTTG GATCTGGAGC TGGTGCTGCT 2641 GTGTAAGGAG AAGCCCACAA CCGCCCTCCT GGACAAGGTG GCCGACAACC TGGCCATCCA 2701 GCTTGCTGCT GTAACAGAAG ACAAGTACGA AATACTGCAA TCTGTCGACG ATGCTGCGAT 2761 TGTGATAAAA AACACAAAAG AGCCTCCATT GTCCCTGACC ATCCACCTGA CATCCCCTGT 2821 TGTCAGAGAA GAAATGGAGA AAGTATTAGC TGGAGAAACG CTATCAGTCA ACGACCCCCC 2881 GGACGTTCTG GACAGGCAGA AATGCCTTGC TGCCTTGGCG TCCCTCCGAC ACGCCAAGTG 2941 GTTCCAGGCC AGAGCCAACG GGCTGAAGTC TTGTGTCATT GTGATCCGGG TCTTGAGGGA 3001 CCTGTGCACT CGCGTGCCCA CCTGGGGTCC CCTCCGAGGC TGGCCTCTCG AGCTCCTGTG 3061 TGAGAAATCC ATTGGCACGG CCAACAGACC GATGGGTGCT GGCGAGGCCC TGCGGAGAGT 3121 GCTGGAGTGC CTGGCGTCGG GCATCGTGAT GCCAGATGGT TCTGGCATTT ATGACCCTTG 3181 TGAAAAAGAA GCCACTGATG CTATTGGGCA TCTAGACAGA CAGCAACGGG AAGATATCAC 3241 ACAGAGTGCG CAGCACGCAC TGCGGCTCGC TGCCTTCGGC CAGCTCCATA AAGTCCTAGG 3301 CATGGACCCT CTGCCTTCCA AGATGCCCAA GAAACCAAAG AATGAAAACC CAGTGGACTA 3361 CACCGTTCAG ATCCCACCAA GCACCACCTA TGCCATTACG CCCATGAAAC GCCCAATGGA 3421 GGAGGACGGG GAGGAGAAGT CGCCCAGCAA AAAGAAGAAG AAGATTCAGA AGAAAGAGGA 3481 GAAGGCAGAG CCCCCCCAGG CTATGAATGC CCTGATGCGG TTGAACCAGC TGAAGCCAGG 3541 GCTGCAGTAC AAGCTGGTGT CCCAGACTGG GCCCGTCCAT GCCCCCATCT TTACCATGTC 3601 TGTGGAGGTT GATGGCAATT CATTCGAGGC CTCTGGGCCC TCCAAAAAGA CGGCCAAGCT 3661 GCACGTGGCC GTTAAGGTGT TACAGGACAT GGGCTTGCCG ACGGGTGCTG AAGGCAGGGA 3721 CTCGAGCAAG GGGGAGGACT CGGCTGAGGA GACCGAGGCG AAGCCAGCAG TGGTGGCCCC 3781 TGCCCCAGTG GTAGAAGCTG TCTCCACCCC TAGTGCGGCC TTTCCCTCAG ATGCCACTGC 3841 CGAGCAGGGG CCGATCCTGA CAAAGCACGG CAAGAACCCA GTCATGGAGC TGAACGAGAA 3901 GAGGCGTGGG CTCAAGTACG AGCTCATCTC CGAGACCGGG GGCAGCCACG ACAAGCGCTT 3961 CGTCATGGAG GTCGAAGTGG ATGGACAGAA GTTCCAAGGT GCTGGTTCCA ACAAAAAGGT 4021 GGCGAAGGCC TACGCTGCTC TTGCTGCCCT AGAAAAGCTT TTCCCTGACA CCCCTCTCGC 4081 CCTTGATGCC AACAAAAAGA AGAGAGCCCC AGTACCCGTC AGAGGGGGAC CGAAATTTGC 4141 TGCTAAGCCA CATAACCCTG GCTTCGGCAT GGGAGGCCCC ATGCACAACG AAGTGCCCCC 4201 ACCCCCCAAC CTTCGAGGGC GGGGAAGAGG CGGGAGCATC CGGGGACGAG GGCGCGGGCG 4261 AGGATTTGGT GGCGCCAACC ATGGAGGCTA CATGAATGCC GGTGCTGGGT ATGGAAGCTA 4321 TGGGTACGGA GGCAACTCGG CGACAGCAGG CTACAGTCAG TTCTACAGCA ACGGAGGGCA 4381 TTCTGGGAAT GCCAGTGGCG GTGGCGGCGG GGGCGGTGGT GGCTCCTCCG GCTATGGCTC 4441 CTACTACCAA GGTGACAACT ACAACTCACC GGTGCCCCCA AAACACGCTG GGAAGAAGCA 4501 GCCGCACGGG GGCCAGCAGA AGCCCTCCTA CGGCTCGGGC TACCAGTCCC ACCAGGGCCA 4561 GCAGCAGTCC TACAACCAGA GCCCCTACAG CAACTATGGC CCTCCACAGG GCAAGCAGAA 4621 AGGCTATAAC CATGGACAAG GCAGCTACTC CTACTCGAAC TCCTACAACT CTCCCGGGGG 4681 CGGGGGCGGA TCCGACTACA ACTACGAGAG CAAATTCAAC TACAGTGGTA GTGGAGGCCG 4741 AAGCGGCGGG AACAGCTACG GCTCAGGCGG GGCATCCTAC AACCCAGGGT CACACGGGGG 4801 CTACGGCGGA GGTTCTGGGG GCGGCTCCTC ATACCAAGGC AAACAAGGAG GCTACTCACA 4861 GTCGAACTAC AACTCCCCGG GGTCCGGCCA GAACTACAGT GGCCCTCCCA GCTCCTACCA 4921 GTCCTCACAA GGCGGCTATG GCAGAAACGC AGACCACAGC ATGAACTACC AGTACAGAGG 4981 ATCCGAACAA AAACTAATAT CAGAGGAAGA TTTGGAACAG AAACTAATCA GTGAAGAAGA 5041 TTTAGAGCAA AAGTTAATTT CCGAGGAGGA CTTATAGCGG CCGCGAAGGA TCTGCGATCG 5101 CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG 5161 AGGGGTCGGC AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA 5221 TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT 5281 AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG 5341 AGGGGCTCGC ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG 5401 GTTGAGTCGC GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA 5461 GGTAAGTTTA AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC 5521 TAGACTCAGC CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT 5581 TTCGTTTTCT GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CGCTAGACGC 5641 CACCATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG 5701 CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCAAGCAGGG 5761 CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT 5821 GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA 5881 CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA 5941 GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG 6001 CGACTTCAAG GTGGTGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT 6061 CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG TGCTGGTGGG 6121 CAGCTTCGCC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTTCG TGGTGGACAG 6181 CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT 6241 CGCCTTCCGC CGCGTGGAGG AGCTGCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA 6301 GCACGCCTTC AAGACCCCCA TCGCCTTCGC CAGATCCCGC GCTCAGTCGT CCAATTCTGC 6361 CGTGGACGGC ACCGCCGGAC CCGGCTCCAC CGGATCTCGC GAGGGCAGAG GAAGTCTTCT 6421 AACATGCGGT GACGTGGAGG AGAATCCCGG CCCTATGACC GAGTACAAGC CCACGGTGCG 6481 CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA 6541 CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT 6601 GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA 6661 CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC 6721 CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT 6781 GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG 6841 CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA 6901 GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC 6961 CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG 7021 CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAAATCAA CCTCTGGATT ACAAAATTTG 7081 TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC 7141 TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA 7201 TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT 7261 GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA 7321 GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC 7381 CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT 7441 GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG 7501 CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG 7561 CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTCCGCCTT CGCCCTCAGA CGAGTCGGAT 7621 CTCCCTTTGG CCGCCTCCCC GCCTGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT 7681 AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG 7741 AAAATAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG 7801 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT 7861 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC 7921 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA 7981 TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG AACTTGTTTA TTGCAGCTTA 8041 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 8101 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGCTCTAGCT 8161 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 8221 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 8281 GGCTTTTTTG GAGGCCTAGA CTTTTGCAGA GACGGCCCAA ATTCGTAATC ATGGTCATAG 8341 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC 8401 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC 8461 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 8521 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG 8581 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 8641 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 8701 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 8761 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 8821 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 8881 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 8941 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 9001 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 9061 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 9121 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA 9181 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 9241 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT 9301 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 9361 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 9421 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 9481 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 9541 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 9601 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT 9661 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 9721 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 9781 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 9841 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 9901 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 9961 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 10021 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 10081 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 10141 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 10201 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 10261 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 10321 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 10381 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 10441 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC 10501 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG 10561 CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT 10621 TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC 10681 GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT 10741 ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG 10801 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC 10861 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC 10921 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.