pLV3-EF1a-ITGA8-3×Myc-Puro pla

pLV3-EF1a-ITGA8-3×Myc-Puro plasmid

PVT86698

pLV3-EF1a-ITGA8-3×Myc-Puro plasmid Information

Plasmid name: pLV3-EF1a-ITGA8(human)-3×Myc-Puro
Alias: NM_003638.3
Gene lenght: 3189bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-EF1a-ITGA8(human)-PGK-Puro
Forward primer:
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-ITGA8-3×Myc-Puro plasmid Description

pLV3-EF1a-ITGA8-3×Myc-Puro This plasmid is suitable for overexpression in mammalian cells. The EF1a strong promoter can initiate the transcription of downstream genes, promoting the C-terminal fusion expression of a 3×Myc tag, facilitating the identification and tracking of the Myc-tagged target protein in experiments such as Western blotting, immunoprecipitation, or immunofluorescence, thereby studying the expression level and interactions of the target protein with other proteins. The plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The plasmid carries a Puro resistance gene for selecting host cells that express the target gene.

pLV3-EF1a-ITGA8-3×Myc-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTTAACTTT 1801 TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG ACATAATAGC 1861 AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTATCGA 1921 TACTAGTAAG GATCTGCGAT CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA 1981 CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC GGGTGCCTAG AGAAGGTGGC 2041 GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG 2101 GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG 2161 CCAGAACACA GCTGAAGCTT CGAGGGGCTC GCATCTCTCC TTCACGCGCC CGCCGCCCTA 2221 CCTGAGGCCG CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC TGTGGTGCCT 2281 CCTGAACTGC GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG GGCCTTTGTC 2341 CGGCGCTCCC TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC CTGACCCTGC 2401 TTGCTCAACT CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA TCCAAGCTGT 2461 GACCGGCGCC TACTCTAGAG CTAGCGAATT CGCCACCATG TCGCCCGGGG CCAGCCGCGG 2521 TCCCCGGGGA AGCCAGGCGC CGCTGATCGC GCCCCTCTGC TGCGCCGCGG CCGCGCTGGG 2581 GATGTTGCTG TGGTCCCCCG CCTGTCAGGC GTTCAACCTG GACGTGGAAA AGCTCACAGT 2641 GTACAGCGGC CCCAAGGGCA GCTACTTCGG CTACGCCGTG GACTTCCACA TACCCGACGC 2701 CCGCACAGCG AGTGTCTTGG TGGGGGCGCC CAAAGCCAAC ACCAGCCAGC CCGATATCGT 2761 GGAAGGGGGA GCCGTCTATT ACTGTCCTTG GCCCGCGGAG GGGTCTGCGC AGTGCAGGCA 2821 GATACCGTTT GACACCACCA ACAACAGAAA GATCAGAGTT AATGGAACCA AAGAACCTAT 2881 CGAGTTCAAA TCCAATCAGT GGTTTGGAGC AACAGTGAAA GCTCACAAAG GAAAAGTTGT 2941 GGCCTGTGCT CCTTTATATC ACTGGAGAAC TCTTAAACCG ACACCAGAAA AGGACCCAGT 3001 TGGCACCTGC TATGTAGCAA TTCAGAACTT CAGCGCCTAT GCCGAGTTCT CTCCTTGCCG 3061 GAACAGCAAT GCTGATCCGG AAGGCCAGGG TTACTGCCAA GCAGGATTTA GTCTGGATTT 3121 TTATAAGAAT GGAGACCTTA TTGTGGGAGG ACCTGGGAGT TTCTACTGGC AAGGACAAGT 3181 GATCACTGCC AGTGTTGCAG ATATCATTGC AAATTACTCA TTCAAGGATA TCCTCAGGAA 3241 ACTGGCAGGA GAAAAGCAGA CGGAAGTGGC TCCAGCTTCC TATGATGACA GTTACCTTGG 3301 ATACTCAGTT GCTGCTGGGG AGTTTACTGG GGATTCTCAG CAAGAATTGG TTGCTGGAAT 3361 TCCAAGAGGA GCACAGAATT TTGGATATGT TTCCATCATT AACTCTACGG ATATGACGTT 3421 TATTCAGAAT TTCACGGGAG AACAGATGGC ATCTTATTTT GGATATACCG TTGTCGTATC 3481 AGATGTTAAC AGTGATGGAC TGGATGATGT CCTGGTTGGG GCACCTCTCT TTATGGAACG 3541 TGAATTTGAG AGCAACCCCA GAGAAGTAGG GCAAATCTAC CTGTATTTGC AAGTGAGCTC 3601 TCTCCTCTTC AGAGACCCCC AGATCCTCAC TGGCACCGAG ACGTTTGGGA GATTCGGTAG 3661 TGCTATGGCA CACTTAGGAG ACCTGAACCA AGATGGATAC AATGACATTG CCATCGGAGT 3721 GCCTTTTGCA GGCAAGGATC AAAGAGGCAA AGTGCTCATT TATAATGGGA ACAAAGATGG 3781 CTTAAACACC AAGCCTTCCC AAGTTCTGCA AGGAGTGTGG GCCTCACATG CTGTCCCTTC 3841 CGGATTTGGC TTTACTTTAA GAGGAGATTC AGACATAGAC AAGAATGATT ACCCAGATTT 3901 GATTGTGGGT GCATTTGGAA CAGGAAAAGT CGCTGTTTAC AGAGCAAGAC CGGTTGTGAC 3961 TGTAGATGCC CAGCTTCTGC TGCACCCAAT GATTATCAAT CTTGAAAATA AAACTTGCCA 4021 GGTTCCAGAC TCTATGACAT CTGCTGCCTG CTTTTCTTTA AGAGTATGTG CATCTGTCAC 4081 AGGCCAGAGC ATTGCAAACA CAATAGTCTT GATGGCAGAG GTGCAATTAG ATTCCCTGAA 4141 ACAGAAAGGA GCTATTAAAC GGACGCTCTT CCTTGATAAC CATCAGGCTC ATCGCGTCTT 4201 CCCTCTTGTG ATAAAAAGGC AGAAATCCCA CCAGTGCCAG GATTTCATCG TTTACCTTCG 4261 AGATGAAACT GAATTCCGAG ATAAATTATC TCCAATCAAC ATTAGTTTGA ATTACAGTTT 4321 GGACGAATCC ACCTTTAAAG AAGGCCTGGA AGTGAAACCA ATATTGAACT ACTACAGAGA 4381 AAACATTGTT AGTGAACAGG CTCACATTCT GGTGGACTGT GGAGAAGACA ATCTGTGTGT 4441 TCCTGACTTG AAGCTGTCGG CTAGACCAGA TAAGCATCAG GTAATCATTG GAGATGAAAA 4501 TCACCTTATG CTCATAATAA ATGCAAGAAA TGAAGGGGAA GGAGCATATG AAGCTGAACT 4561 CTTTGTAATG ATACCAGAAG AGGCAGATTA TGTTGGAATC GAACGCAACA ACAAGGGATT 4621 TCGACCACTG AGCTGTGAGT ACAAGATGGA AAATGTAACC AGGATGGTGG TGTGTGACCT 4681 TGGGAACCCT ATGGTGTCTG GAACAAATTA TTCCCTGGGC CTCCGATTTG CAGTTCCACG 4741 TCTTGAGAAA ACAAACATGA GCATTAACTT CGATCTCCAA ATCAGAAGTT CCAACAAGGA 4801 CAATCCAGAC AGCAATTTTG TGAGCCTGCA AATCAACATC ACTGCTGTAG CGCAGGTGGA 4861 AATAAGAGGA GTGTCACACC CTCCGCAGAT TGTTCTGCCC ATTCATAACT GGGAACCAGA 4921 AGAGGAGCCC CACAAAGAGG AGGAGGTTGG ACCATTGGTG GAACATATTT ATGAGCTGCA 4981 CAATATTGGA CCAAGTACCA TCAGTGACAC CATCCTGGAG GTGGGCTGGC CTTTCTCTGC 5041 CCGGGATGAA TTTCTTCTCT ATATTTTCCA TATTCAAACT CTGGGACCTC TGCAGTGCCA 5101 ACCAAATCCT AATATCAATC CACAGGATAT AAAGCCTGCT GCCTCCCCAG AGGACACCCC 5161 TGAGCTCAGC GCCTTTTTGC GAAACTCTAC TATTCCTCAT CTTGTCAGGA AGAGGGATGT 5221 ACATGTGGTC GAATTCCACA GACAGAGCCC TGCAAAAATA CTGAATTGTA CAAATATCGA 5281 GTGTTTACAA ATCTCCTGTG CAGTGGGACG ACTCGAAGGA GGAGAAAGCG CAGTCCTGAA 5341 AGTCAGGTCA CGATTATGGG CCCACACCTT CCTCCAGAGA AAAAATGATC CCTATGCTCT 5401 TGCATCCCTG GTGTCCTTTG AAGTTAAGAA GATGCCTTAT ACAGATCAGC CAGCAAAACT 5461 CCCAGAAGGA AGCATAGTAA TTAAGACATC AGTTATTTGG GCAACTCCGA ATGTTTCCTT 5521 CTCAATCCCA TTATGGGTAA TAATACTAGC AATACTTCTT GGATTGTTGG TTCTCGCCAT 5581 TTTAACCTTA GCTTTATGGA AGTGTGGATT CTTTGACAGA GCCAGACCTC CTCAGGAGGA 5641 CATGACCGAC AGGGAACAGC TGACAAATGA CAAGACCCCT GAGGCACTCG AGGAACAAAA 5701 ACTAATATCA GAGGAAGATT TGGAACAGAA ACTAATCAGT GAAGAAGATT TAGAGCAAAA 5761 GTTAATTTCC GAGGAGGACT TATAGGCGGC CGCTGGGTAG GGGAGGCGCT TTTCCCAAGG 5821 CAGTCTGGAG CATGCGCTTT AGCAGCCCCG CTGGGCACTT GGCGCTACAC AAGTGGCCTC 5881 TGGCCTCGCA CACATTCCAC ATCCACCGGT AGGCGCCAAC CGGCTCCGTT CTTTGGTGGC 5941 CCCTTCGCGC CACCTTCTAC TCCTCCCCTA GTCAGGAAGT TCCCCCCCGC CCCGCAGCTC 6001 GCGTCGTGCA GGACGTGACA AATGGAAGTA GCACGTCTCA CTAGTCTCGT GCAGATGGAC 6061 AGCACCGCTG AGCAATGGAA GCGGGTAGGC CTTTGGGGCA GCGGCCAATA GCAGCTTTGC 6121 TCCTTCGCTT TCTGGGCTCA GAGGCTGGGA AGGGGTGGGT CCGGGGGCGG GCTCAGGGGC 6181 GGGCTCAGGG GCGGGGCGGG CGCCCGAAGG TCCTCCGGAG GCCCGGCATT CTGCACGCTT 6241 CAAAAGCGCA CGTCTGCCGC GCTGTTCTCC TCTTCCTCAT CTCCGGGCCT TTCGACCTGC 6301 AGCCCAAGCT TACCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG 6361 TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA 6421 CCGTCGATCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC 6481 GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT 6541 GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG 6601 CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC 6661 ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG 6721 GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG 6781 TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT 6841 TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA 6901 AGCCCGGTGC CTGAGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 6961 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 7021 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 7081 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 7141 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 7201 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 7261 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT 7321 CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 7381 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 7441 TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG 7501 CCTCCCCGCC TGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC 7561 TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAA ATAAGATCTG 7621 CTTTTTGCTT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC 7681 TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG 7741 TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG 7801 TGGAAAATCT CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA 7861 AAGAAATGAA TATCAGAGAG TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA 7921 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT 7981 GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA 8041 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG 8101 AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG 8161 GCCTAGACTT TTGCAGAGAC GGCCCAAATT CGTAATCATG GTCATAGCTG TTTCCTGTGT 8221 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 8281 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 8341 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 8401 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 8461 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 8521 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 8581 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 8641 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 8701 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 8761 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 8821 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 8881 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 8941 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 9001 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT 9061 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 9121 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 9181 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 9241 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 9301 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 9361 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 9421 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 9481 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 9541 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 9601 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 9661 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 9721 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 9781 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 9841 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 9901 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 9961 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 10021 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 10081 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 10141 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 10201 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 10261 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 10321 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA 10381 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG 10441 ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG 10501 ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT 10561 GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA 10621 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC 10681 GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG 10741 GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT 10801 GTAAAACGAC GGCCAGTGCC AAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.