pLV3-CMV-COL27A1-3×FLAG-CopGFP

pLV3-CMV-COL27A1-3×FLAG-CopGFP-Puro plasmid

PVT86660

pLV3-CMV-COL27A1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-COL27A1(human)-3×FLAG-CopGFP-Puro
Alias: NM_032888.4;STLS
Gene lenght: 5580bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CPPT-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-COL27A1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-COL27A1-3×FLAG-CopGFP-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of downstream genes, promoting the C-terminal fusion expression of a 3×FLAG tag, which facilitates detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The plasmid carries the CopGFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid also carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV3-CMV-COL27A1-3×FLAG-CopGFP-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 1921 TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 1981 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 2041 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 2101 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 2161 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA 2221 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC 2281 TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGG GAGCGGGATC GGCGCGGGGG 2341 GCCCGAGGCA CAGCGGCGGC GGCGGCGGCG CGCGGGGGGG GGTTTCTCTT CTCCTGGATC 2401 TTAGTCTCGT TTGCCTGTCA CCTGGCCTCC ACCCAAGGAG CTCCTGAAGA TGTGGACATC 2461 CTCCAGCGGC TGGGCCTCAG CTGGACGAAG GCCGGGAGCC CTGCACCCCC GGGAGTCATT 2521 CCTTTCCAGT CGGGCTTCAT CTTTACGCAG CGGGCCCGGC TCCAGGCTCC CACGGGCACC 2581 GTCATTCCTG CCGCCTTGGG CACAGAGCTG GCACTGGTGC TGAGCCTCTG CTCCCACCGG 2641 GTGAACCATG CCTTCCTCTT CGCTGTCCGC AGCCAGAAAC GCAAGCTGCA GCTGGGCCTG 2701 CAGTTCCTCC CCGGCAAGAC GGTCGTCCAC CTCGGGTCCC GGCGCTCAGT GGCCTTCGAC 2761 CTCGACATGC ACGACGGGCG CTGGCACCAC CTGGCCCTCG AGCTCCGAGG CCGCACAGTC 2821 ACTCTGGTGA CTGCCTGCGG GCAGCGCCGG GTGCCTGTCC TGCTGCCTTT CCACAGGGAC 2881 CCTGCACTCG ACCCTGGGGG CTCCTTCCTC TTTGGGAAGA TGAACCCGCA TGCAGTCCAG 2941 TTTGAAGGTG CTCTCTGCCA GTTCAGTATC TACCCTGTGA CGCAGGTCGC TCACAATTAC 3001 TGTACCCACC TGAGGAAGCA GTGTGGACAG GCTGACACGT ACCAGTCCCC ACTGGGACCT 3061 CTCTTCTCCC AAGACTCTGG CAGACCTTTT ACCTTCCAGT CCGACCTCGC CCTGCTAGGC 3121 CTGGAGAACT TGACCACTGC CACACCAGCC CTGGGGTCAC TGCCAGCAGG CAGGGGACCC 3181 AGGGGGACTG TGGCACCCGC CACGCCCACC AAGCCCCAAA GGACTAGCCC CACAAACCCT 3241 CACCAGCATA TGGCGGTGGG AGGCCCAGCC CAAACCCCGC TGCTACCTGC CAAGCTGTCA 3301 GCCAGTAACG CACTTGATCC CATGCTCCCA GCCTCTGTTG GCGGCTCTAC CAGAACGCCT 3361 CGCCCTGCGG CCGCTCAACC ATCACAGAAG ATCACAGCCA CCAAAATCCC CAAAAGCCTC 3421 CCTACCAAGC CTTCGGCCCC TTCTACTTCA ATTGTGCCCA TCAAAAGCCC CCATCCTACC 3481 CAGAAAACAG CTCCATCTTC ATTTACAAAG TCAGCCCTAC CCACTCAGAA GCAAGTGCCA 3541 CCTACTTCCC GTCCAGTTCC TGCCAGAGTC TCCCGTCCCG CAGAGAAGCC CATCCAGAGG 3601 AACCCGGGAA TGCCCAGGCC CCCACCGCCC AGCACCCGGC CCCTACCTCC TACCACCAGC 3661 TCCTCTAAAA AACCCATTCC CACACTAGCT CGGACTGAGG CCAAGATAAC CAGCCATGCC 3721 AGTAAGCCGG CCTCTGCCCG CACCAGCACC CACAAACCTC CCCCATTTAC TGCTTTATCC 3781 TCATCTCCTG CCCCTACTCC TGGTTCTACC AGGAGTACTC GGCCACCAGC CACGATGGTA 3841 CCTCCAACTT CGGGCACCAG CACTCCCAGA ACAGCACCTG CCGTCCCCAC TCCTGGCTCA 3901 GCTCCCACTG GAAGCAAGAA GCCCATTGGA TCGGAAGCCT CAAAGAAAGC CGGACCCAAG 3961 AGCAGCCCCC GGAAGCCTGT CCCCCTCAGA CCTGGGAAGG CAGCCAGGGA TGTCCCCTTG 4021 AGCGATCTGA CAACCAGGCC TAGCCCCAGA CAGCCCCAGC CCAGTCAGCA GACCACCCCG 4081 GCCCTGGTAT TGGCCCCGGC GCAATTCCTG TCCTCCAGCC CCCGGCCCAC GAGCAGTGGC 4141 TATTCGATCT TCCACCTGGC AGGATCTACG CCTTTCCCTC TGCTGATGGG GCCTCCGGGA 4201 CCCAAGGGAG ACTGTGGCTT GCCGGGTCCC CCTGGGCTAC CTGGGCTACC TGGAATCCCT 4261 GGTGCACGTG GGCCTCGGGG TCCTCCTGGG CCTTATGGAA ATCCAGGTCT CCCCGGCCCT 4321 CCTGGAGCCA AAGGACAGAA AGGGGACCCA GGGCTCTCAC CAGGAAAGGC CCACGATGGG 4381 GCAAAGGGTG ACATGGGCTT GCCTGGGCTC TCCGGGAATC CAGGACCTCC GGGACGAAAG 4441 GGACACAAGG GCTATCCTGG ACCGGCAGGG CACCCCGGAG AACAGGGGCA GCCAGGACCT 4501 GAGGGCAGCC CAGGGGCCAA AGGTTACCCT GGCAGGCAGG GGTTACCTGG ACCGGTAGGA 4561 GATCCCGGCC CCAAAGGCAG CAGGGGCTAC ATTGGGCTCC CAGGGCTCTT CGGCCTGCCA 4621 GGGTCTGATG GAGAACGAGG CCTGCCTGGC GTTCCTGGCA AGAGGGGCAA GATGGGTATG 4681 CCGGGGTTTC CTGGAGTCTT TGGGGAAAGA GGCCCTCCTG GACTGGATGG AAATCCTGGA 4741 GAACTGGGCC TGCCAGGCCC CCCTGGAGTC CCCGGCCTCA TTGGTGACTT AGGAGTGTTG 4801 GGTCCGATTG GCTACCCGGG ACCCAAGGGC ATGAAGGGAC TGATGGGCAG CGTGGGGGAG 4861 CCCGGACTGA AAGGTGATAA GGGTGAACAA GGGGTTCCAG GTGTGTCAGG AGATCCCGGA 4921 TTCCAAGGAG ACAAGGGGAG CCAGGGGTTG CCAGGGTTCC CCGGTGCACG GGGGAAGCCA 4981 GGGCCTCTGG GCAAAGTCGG AGACAAAGGA TCCATTGGGT TTCCCGGGCC CCCTGGACCC 5041 GAGGGATTCC CAGGAGACAT CGGCCCCCCT GGCGACAATG GCCCAGAAGG CATGAAGGGT 5101 AAGCCTGGAG CCCGAGGCCT GCCGGGACCC CGTGGGCAGC TGGGGCCCGA GGGAGATGAG 5161 GGACCCATGG GGCCGCCAGG GGCCCCTGGC TTGGAGGGTC AGCCTGGCAG GAAGGGGTTT 5221 CCTGGGAGGC CCGGCCTGGA TGGCGTGAAG GGGGAACCAG GGGATCCTGG TCGGCCGGGG 5281 CCTGTGGGAG AGCAGGGATT TATGGGATTC ATTGGTCTGG TCGGGGAGCC AGGAATCGTG 5341 GGAGAAAAGG GTGATCGTGG CATGATGGGA CCCCCAGGCG TGCCTGGACC CAAGGGGTCG 5401 ATGGGTCATC CTGGAATGCC AGGTGGTATG GGGACCCCTG GAGAGCCTGG ACCCCAGGGT 5461 CCTCCAGGAT CTCGAGGCCC ACCAGGCATG AGGGGAGCAA AGGGACGTCG GGGCCCCCGA 5521 GGACCGGACG GACCAGCTGG GGAGCAAGGG TCCAGGGGCC TGAAGGGCCC TCCAGGACCC 5581 CAGGGCAGAC CGGGCCGGCC TGGACAGCAG GGTGTGGCTG GTGAGCGAGG CCACTTGGGC 5641 TCGAGAGGCT TTCCTGGCAT CCCGGGTCCC TCAGGCCCCC CAGGCACCAA GGGCCTCCCA 5701 GGAGAACCGG GCCCTCAGGG ACCCCAGGGG CCAATTGGGC CTCCAGGAGA GATGGGACCC 5761 AAGGGGCCGC CTGGTGCAGT GGGAGAACCG GGCCTTCCTG GGGAAGCCGG GATGAAGGGT 5821 GACCTTGGAC CCCTGGGCAC TCCTGGGGAG CAGGGCCTCA TTGGGCAACG GGGAGAGCCA 5881 GGCCTTGAGG GTGACAGTGG CCCCATGGGA CCTGATGGGC TGAAGGGGGA CAGGGGAGAC 5941 CCAGGGCCTG ATGGAGAACA TGGCGAGAAA GGCCAGGAAG GGCTGATGGG TGAGGACGGG 6001 CCCCCCGGCC CCCCTGGCGT CACTGGTGTC CGGGGTCCTG AAGGAAAATC AGGGAAGCAA 6061 GGCGAGAAGG GCCGCACTGG AGCCAAGGGT GCCAAGGGCT ATCAAGGACA GCTGGGTGAG 6121 ATGGGCGTCC CTGGAGACCC TGGACCCCCT GGCACTCCAG GCCCTAAAGG GTCCCGGGGC 6181 AGCCTGGGAC CAACGGGTGC TCCGGGACGC ATGGGGGCCC AAGGAGAACC GGGACTGGCT 6241 GGTTATGATG GACACAAAGG CATTGTGGGA CCCCTTGGAC CTCCTGGACC AAAAGGCGAA 6301 AAGGGGGAGC AGGGCGAGGA CGGCAAGGCT GAGGGGCCCC CTGGGCCACC TGGAGATCGG 6361 GGCCCTGTGG GTGATCGAGG AGACCGCGGG GAACCGGGAG ACCCTGGGTA CCCTGGACAG 6421 GAGGGTGTGC AAGGCCTCCG TGGAAAGCCA GGCCAGCAGG GCCAACCCGG GCATCCGGGA 6481 CCCCGGGGGT GGCCGGGACC CAAAGGATCG AAAGGCGCAG AGGGACCAAA GGGAAAGCAA 6541 GGCAAGGCAG GGGCCCCAGG CCGGAGGGGG GTCCAGGGCC TGCAGGGGCT GCCAGGGCCC 6601 CGGGGCGTGG TGGGGAGACA GGGCCTCGAG GGCATCGCTG GACCAGATGG GCTTCCTGGC 6661 AGGGACGGGC AAGCAGGACA GCAGGGGGAG CAGGGAGACG ATGGGGACCC TGGCCCCATG 6721 GGCCCTGCTG GGAAGAGAGG AAATCCAGGT GTGGCCGGCT TACCTGGAGC ACAGGGACCC 6781 CCAGGATTCA AGGGTGAGAG TGGGTTACCC GGACAGCTGG GTCCCCCTGG CAAGCGAGGA 6841 ACAGAGGGCA GAACGGGGCT CCCTGGAAAC CAGGGGGAGC CTGGGTCCAA AGGCCAGCCG 6901 GGCGACTCTG GCGAGATGGG CTTCCCAGGA ATGGCAGGTC TCTTCGGACC CAAGGGCCCG 6961 CCTGGAGACA TTGGCTTCAA AGGCATCCAG GGCCCTCGGG GGCCACCTGG CTTGATGGGA 7021 AAGGAAGGCA TCGTCGGGCC CCTCGGAATC CTGGGACCTT CGGGACTCCC GGGTCCGAAG 7081 GGTGACAAAG GCAGCCGTGG GGACTGGGGA TTGCAAGGTC CGAGGGGTCC TCCCGGCCCC 7141 AGAGGGCGGC CCGGCCCCCC GGGTCCTCCA GGGGGTCCTA TCCAATTGCA ACAAGATGAT 7201 CTTGGGGCAG CTTTCCAGAC GTGGATGGAC ACCAGTGGAG CACTCAGGCC AGAGAGTTAC 7261 AGCTATCCAG ACCGGCTGGT GCTGGACCAG GGAGGAGAGA TCTTTAAAAC CTTACACTAC 7321 CTCAGCAACC TCATCCAGAG CATTAAGACG CCCCTGGGCA CCAAAGAGAA CCCCGCCCGG 7381 GTCTGCAGGG ACCTCATGGA CTGTGAGCAG AAGATGGTGG ATGGTACCTA CTGGGTGGAT 7441 CCAAACCTTG GCTGCTCCTC TGACACCATC GAGGTCTCCT GCAACTTCAC TCATGGTGGA 7501 CAGACGTGTC TCAAGCCCAT CACGGCCTCC AAGGTCGAGT TTGCCATCAG CCGGGTCCAG 7561 ATGAATTTCC TGCACCTGCT AAGCTCCGAG GTGACCCAGC ACATCACCAT CCACTGCCTT 7621 AACATGACCG TGTGGCAGGA GGGCACTGGG CAGACCCCAG CCAAGCAGGC CGTACGCTTC 7681 CGGGCCTGGA ATGGACAGAT TTTTGAAGCT GGGGGTCAGT TCCGGCCCGA GGTGTCCATG 7741 GATGGCTGCA AGGTCCAAGA TGGCCGCTGG CATCAGACAC TCTTCACCTT CCGGACCCAA 7801 GACCCCCAAC AGCTGCCCAT CATCAGTGTG GACAACCTCC CTCCTGCCTC ATCAGGGAAG 7861 CAGTACCGCC TGGAAGTTGG ACCTGCGTGC TTCCTCGGAT CCGACTACAA AGACCATGAC 7921 GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GCGGCCGCGA 7981 AGGATCTGCG ATCGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC 8041 GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA 8101 AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG 8161 TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA 8221 CAGCTGAAGC TTCGAGGGGC TCGCATCTCT CCTTCACGCG CCCGCCGCCC TACCTGAGGC 8281 CGCCATCCAC GCCGGTTGAG TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC CTCCTGAACT 8341 GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC 8401 CCTTGGAGCC TACCTAGACT CAGCCGGCTC TCCACGCTTT GCCTGACCCT GCTTGCTCAA 8461 CTCTACGTCT TTGTTTCGTT TTCTGTTCTG CGCCGTTACA GATCCAAGCT GTGACCGGCG 8521 CCTACGCTAG ACGCCACCAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG 8581 TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC 8641 ACCCCCAAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC 8701 AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC 8761 AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC 8821 ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC 8881 GGCCGCGTGA TCGGCGACTT CAAGGTGGTG GGCACCGGCT TCCCCGAGGA CAGCGTGATC 8941 TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT 9001 AACGTGCTGG TGGGCAGCTT CGCCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC 9061 TTCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC 9121 GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGCTGC ACAGCAACAC CGAGCTGGGC 9181 ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCCATCGCCT TCGCCAGATC CCGCGCTCAG 9241 TCGTCCAATT CTGCCGTGGA CGGCACCGCC GGACCCGGCT CCACCGGATC TCGCGAGGGC 9301 AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC CCGGCCCTAT GACCGAGTAC 9361 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC 9421 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG 9481 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG 9541 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG 9601 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC 9661 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC 9721 CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG 9781 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG 9841 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG 9901 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAAA TCAACCTCTG 9961 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 10021 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 10081 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 10141 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 10201 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 10261 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 10321 AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC 10381 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 10441 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 10501 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GTACCTTTAA GACCAATGAC 10561 TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT 10621 AATTCACTCC CAACGAAGAT AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA 10681 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 10741 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 10801 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA 10861 TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT 10921 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA 10981 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA 11041 TGTCTGGCTC TAGCTATCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 11101 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 11161 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGACTTT 11221 TGCAGAGACC AAATTCGTAA TCATGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT 11281 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG 11341 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT 11401 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG 11461 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC 11521 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 11581 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 11641 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 11701 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 11761 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 11821 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 11881 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 11941 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 12001 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 12061 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 12121 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 12181 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 12241 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 12301 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 12361 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 12421 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 12481 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 12541 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 12601 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 12661 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 12721 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 12781 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 12841 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 12901 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 12961 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 13021 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 13081 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 13141 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 13201 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 13261 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 13321 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 13381 CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA 13441 TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG 13501 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA 13561 AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC 13621 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT 13681 AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG 13741 GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG 13801 GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG 13861 TGCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.