pLV3-CMV-MAPT-2-3×FLAG-CopGFP-

pLV3-CMV-MAPT-2-3×FLAG-CopGFP-Puro plasmid

PVT86131

pLV3-CMV-MAPT-2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MAPT(human)-2-3×FLAG-CopGFP-Puro
Alias: NM_001123066.4;DDPAC; FTD1; FTDP-17; MAPTL; MSTD; MTBT1;MTBT2; PPND; PPP1R103; TAU; tau-40; Tau-PHF6;
Gene lenght: 2328bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MAPT-2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MAPT-2-3×FLAG-CopGFP-Puro This plasmid is suitable for the overexpression of mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of the downstream human MAPT gene, promoting the expression of the MAPT C-terminal fusion with a 3×FLAG tag, which facilitates the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, making it easier for stable integration of the gene into host cells. The plasmid also carries the CopGFP green fluorescent protein for observing the expression and localization of the target gene. Furthermore, the plasmid contains a Puro resistance gene for selecting host cells that express the target gene.

pLV3-CMV-MAPT-2-3×FLAG-CopGFP-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 1921 TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 1981 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 2041 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 2101 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 2161 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA 2221 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC 2281 TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGG CTGAGCCCCG CCAGGAGTTC 2341 GAAGTGATGG AAGATCACGC TGGGACGTAC GGGTTGGGGG ACAGGAAAGA TCAGGGGGGC 2401 TACACCATGC ACCAAGACCA AGAGGGTGAC ACGGACGCTG GCCTGAAAGA ATCTCCCCTG 2461 CAGACCCCCA CTGAGGACGG ATCTGAGGAA CCGGGCTCTG AAACCTCTGA TGCTAAGAGC 2521 ACTCCAACAG CGGAAGATGT GACAGCACCC TTAGTGGATG AGGGAGCTCC CGGCAAGCAG 2581 GCTGCCGCGC AGCCCCACAC GGAGATCCCA GAAGGAACCA CAGCTGAAGA AGCAGGCATT 2641 GGAGACACCC CCAGCCTGGA AGACGAAGCT GCTGGTCACG TGACCCAAGA GCCTGAAAGT 2701 GGTAAGGTGG TCCAGGAAGG CTTCCTCCGA GAGCCAGGCC CCCCAGGTCT GAGCCACCAG 2761 CTCATGTCCG GCATGCCTGG GGCTCCCCTC CTGCCTGAGG GCCCCAGAGA GGCCACACGC 2821 CAACCTTCGG GGACAGGACC TGAGGACACA GAGGGCGGCC GCCACGCCCC TGAGCTGCTC 2881 AAGCACCAGC TTCTAGGAGA CCTGCACCAG GAGGGGCCGC CGCTGAAGGG GGCAGGGGGC 2941 AAAGAGAGGC CGGGGAGCAA GGAGGAGGTG GATGAAGACC GCGACGTCGA TGAGTCCTCC 3001 CCCCAAGACT CCCCTCCCTC CAAGGCCTCC CCAGCCCAAG ATGGGCGGCC TCCCCAGACA 3061 GCCGCCAGAG AAGCCACCAG CATCCCAGGC TTCCCAGCGG AGGGTGCCAT CCCCCTCCCT 3121 GTGGATTTCC TCTCCAAAGT TTCCACAGAG ATCCCAGCCT CAGAGCCCGA CGGGCCCAGT 3181 GTAGGGCGGG CCAAAGGGCA GGATGCCCCC CTGGAGTTCA CGTTTCACGT GGAAATCACA 3241 CCCAACGTGC AGAAGGAGCA GGCGCACTCG GAGGAGCATT TGGGAAGGGC TGCATTTCCA 3301 GGGGCCCCTG GAGAGGGGCC AGAGGCCCGG GGCCCCTCTT TGGGAGAGGA CACAAAAGAG 3361 GCTGACCTTC CAGAGCCCTC TGAAAAGCAG CCTGCTGCTG CTCCGCGGGG GAAGCCCGTC 3421 AGCCGGGTCC CTCAACTCAA AGCTCGCATG GTCAGTAAAA GCAAAGACGG GACTGGAAGC 3481 GATGACAAAA AAGCCAAGAC ATCCACACGT TCCTCTGCTA AAACCTTGAA AAATAGGCCT 3541 TGCCTTAGCC CCAAACACCC CACTCCTGGT AGCTCAGACC CTCTGATCCA ACCCTCCAGC 3601 CCTGCTGTGT GCCCAGAGCC ACCTTCCTCT CCTAAATACG TCTCTTCTGT CACTTCCCGA 3661 ACTGGCAGTT CTGGAGCAAA GGAGATGAAA CTCAAGGGGG CTGATGGTAA AACGAAGATC 3721 GCCACACCGC GGGGAGCAGC CCCTCCAGGC CAGAAGGGCC AGGCCAACGC CACCAGGATT 3781 CCAGCAAAAA CCCCGCCCGC TCCAAAGACA CCACCCAGCT CTGCGACTAA GCAAGTCCAG 3841 AGAAGACCAC CCCCTGCAGG GCCCAGATCT GAGAGAGGTG AACCTCCAAA ATCAGGGGAT 3901 CGCAGCGGCT ACAGCAGCCC CGGCTCCCCA GGCACTCCCG GCAGCCGCTC CCGCACCCCG 3961 TCCCTTCCAA CCCCACCCAC CCGGGAGCCC AAGAAGGTGG CAGTGGTCCG TACTCCACCC 4021 AAGTCGCCGT CTTCCGCCAA GAGCCGCCTG CAGACAGCCC CCGTGCCCAT GCCAGACCTG 4081 AAGAATGTCA AGTCCAAGAT CGGCTCCACT GAGAACCTGA AGCACCAGCC GGGAGGCGGG 4141 AAGGTGCAGA TAATTAATAA GAAGCTGGAT CTTAGCAACG TCCAGTCCAA GTGTGGCTCA 4201 AAGGATAATA TCAAACACGT CCCGGGAGGC GGCAGTGTGC AAATAGTCTA CAAACCAGTT 4261 GACCTGAGCA AGGTGACCTC CAAGTGTGGC TCATTAGGCA ACATCCATCA TAAACCAGGA 4321 GGTGGCCAGG TGGAAGTAAA ATCTGAGAAG CTTGACTTCA AGGACAGAGT CCAGTCGAAG 4381 ATTGGGTCCC TGGACAATAT CACCCACGTC CCTGGCGGAG GAAATAAAAA GATTGAAACC 4441 CACAAGCTGA CCTTCCGCGA GAACGCCAAA GCCAAGACAG ACCACGGGGC GGAGATCGTG 4501 TACAAGTCGC CAGTGGTGTC TGGGGACACG TCTCCACGGC ATCTCAGCAA TGTCTCCTCC 4561 ACCGGCAGCA TCGACATGGT AGACTCGCCC CAGCTCGCCA CGCTAGCTGA CGAGGTGTCT 4621 GCCTCCCTGG CCAAGCAGGG TTTGGGATCC GACTACAAAG ACCATGACGG TGATTATAAA 4681 GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGC GGCCGCGAAG GATCTGCGAT 4741 CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG 4801 GGAGGGGTCG GCAATTGAAC GGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT 4861 GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA 4921 GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GCTGAAGCTT 4981 CGAGGGGCTC GCATCTCTCC TTCACGCGCC CGCCGCCCTA CCTGAGGCCG CCATCCACGC 5041 CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC TGTGGTGCCT CCTGAACTGC GTCCGCCGTC 5101 TAGGTAAGTT TAAAGCTCAG GTCGAGACCG GGCCTTTGTC CGGCGCTCCC TTGGAGCCTA 5161 CCTAGACTCA GCCGGCTCTC CACGCTTTGC CTGACCCTGC TTGCTCAACT CTACGTCTTT 5221 GTTTCGTTTT CTGTTCTGCG CCGTTACAGA TCCAAGCTGT GACCGGCGCC TACGCTAGAC 5281 GCCACCATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC 5341 GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCAAGCAG 5401 GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG 5461 CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG 5521 AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC 5581 GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC 5641 GGCGACTTCA AGGTGGTGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG 5701 ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGTGCTGGTG 5761 GGCAGCTTCG CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTT CGTGGTGGAC 5821 AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG 5881 TTCGCCTTCC GCCGCGTGGA GGAGCTGCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC 5941 CAGCACGCCT TCAAGACCCC CATCGCCTTC GCCAGATCCC GCGCTCAGTC GTCCAATTCT 6001 GCCGTGGACG GCACCGCCGG ACCCGGCTCC ACCGGATCTC GCGAGGGCAG AGGAAGTCTT 6061 CTAACATGCG GTGACGTGGA GGAGAATCCC GGCCCTATGA CCGAGTACAA GCCCACGGTG 6121 CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC 6181 GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG 6241 CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC 6301 GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC 6361 GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG 6421 ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC 6481 GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG 6541 GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC 6601 CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG 6661 CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGAAATC AACCTCTGGA TTACAAAATT 6721 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 6781 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 6841 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 6901 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 6961 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 7021 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 7081 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 7141 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 7201 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 7261 ATCTCCCTTT GGGCCGCCTC CCCGCCTGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC 7321 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA 7381 ACGAAGATAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC 7441 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG 7501 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG 7561 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA 7621 GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC 7681 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 7741 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA 7801 GCTATCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 7861 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 7921 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGACTTTTG CAGAGACCAA 7981 ATTCGTAATC ATGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 8041 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 8101 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 8161 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 8221 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 8281 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 8341 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 8401 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 8461 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 8521 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 8581 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 8641 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 8701 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 8761 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 8821 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 8881 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 8941 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 9001 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 9061 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 9121 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 9181 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 9241 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 9301 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 9361 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 9421 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 9481 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 9541 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 9601 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 9661 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 9721 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 9781 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 9841 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 9901 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 9961 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 10021 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 10081 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 10141 ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC 10201 GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT 10261 CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG 10321 CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT 10381 TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA 10441 CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG 10501 GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG 10561 GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG CCAAGCTG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.