pCMV-EGFP-HA-NLRP10-Neo plasmi

pCMV-EGFP-HA-NLRP10-Neo plasmid

PVT86119

pCMV-EGFP-HA-NLRP10-Neo plasmid Information

Plasmid name: pCMV-EGFP-HA-NLRP10(human)-Neo
Alias: NM_001391958.1;CLR11.1; NALP10; NOD8; PAN5; PYNOD
Gene lenght: 1968bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: pUC
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-EGFP-3×Linker-MCS-Neo
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-HA-NLRP10-Neo plasmid Description

pCMV-EGFP-HA-NLRP10-Neo This plasmid is suitable for overexpression in mammalian cells. The CMV enhancer and CMV promoter carried by the plasmid can jointly initiate the efficient expression of the downstream human NLRP10 gene. The plasmid carries the EGFP green fluorescent protein at the N-terminus of the CYP3A4 gene, allowing for visual observation of the expression location and level of the target gene, and fuses an HA tag at the N-terminus, facilitating experiments such as protein immunoprecipitation and co-immunoprecipitation. The plasmid carries a Neo/G418 resistance gene for screening host cells expressing the target gene.

pCMV-EGFP-HA-NLRP10-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC 901 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC 961 GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC 1021 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG 1081 CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG 1141 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC 1201 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC 1261 GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC 1321 AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC 1381 CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC 1441 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC 1501 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG 1561 ATCACTCTCG GCATGGACGA GCTGTACAAG GGAGGCGGAG GTAGTGGAGG AGGGGGAAGC 1621 GGAGGCGGAG GGAGTGAATT CTACCCATAC GATGTTCCAG ATTACGCTGG ATCCATGGCC 1681 ATGGCCAAGG CCAGAAAGCC CCGGGAGGCA TTGCTCTGGG CCTTGAGTGA CCTTGAGGAG 1741 AACGATTTCA AGAAGTTAAA GTTCTACTTA CGGGATATGA CCCTGTCTGA GGGCCAGCCC 1801 CCACTGGCCA GAGGGGAGTT GGAGGGCCTG ATTCCGGTGG ACCTGGCAGA ATTACTGATT 1861 TCAAAGTATG GAGAAAAGGA GGCTGTGAAA GTTGTCCTCA AGGGCTTGAA GGTCATGAAC 1921 CTGTTGGAAC TTGTGGACCA GCTCAGCCAT ATTTGTCTGC ATGATTACAG AGAAGTATAC 1981 CGAGAGCATG TGCGCTGCCT AGAGGAATGG CAGGAAGCAG GAGTCAATGG CAGATACAAC 2041 CAGGTGCTCC TGGTGGCCAA GCCCAGCTCA GAGAGCCCAG AATCACTTGC CTGCCCCTTC 2101 CCGGAGCAGG AGCTGGAGTC TGTCACGGTG GAGGCTCTAT TTGATTCAGG GGAAAAGCCC 2161 TCACTGGCCC CATCCTTAGT TGTGCTACAG GGGTCGGCTG GCACTGGAAA GACAACTCTC 2221 GCCAGAAAAA TGGTGTTGGA CTGGGCCACC GGTACTCTGT ACCCAGGCCG GTTTGATTAT 2281 GTCTTTTATG TAAGCTGCAA AGAAGTGGTC CTGCTGCTGG AGAGCAAACT GGAGCAGCTC 2341 CTTTTCTGGT GCTGCGGGGA CAATCAAGCC CCTGTCACAG AGATTCTGAG GCAGCCAGAG 2401 CGGCTCCTGT TCATCCTGGA TGGCTTTGAT GAGCTGCAGA GGCCCTTTGA AGAAAAGTTG 2461 AAGAAGAGGG GTTTGAGTCC CAAGGAGAGC CTGCTGCACC TTCTAATTAG GAGACATACA 2521 CTCCCCACGT GCTCCCTTCT CATCACCACC CGGCCCCTGG CTTTGAGGAA TCTGGAGCCC 2581 TTGCTGAAAC AAGCACGTCA TGTCCATATC CTAGGCTTCT CTGAGGAGGA GAGGGCGAGG 2641 TACTTCAGCT CCTATTTCAC GGATGAGAAG CAAGCTGACC GTGCCTTCGA CATTGTACAG 2701 AAAAATGACA TTCTCTACAA AGCGTGTCAG GTTCCAGGCA TTTGCTGGGT GGTCTGCTCC 2761 TGGCTGCAGG GGCAGATGGA GAGAGGCAAA GTTGTCTTAG AGACACCTAG AAACAGCACT 2821 GACATCTTCA TGGCTTACGT CTCCACCTTT CTGCCGCCCG ATGATGATGG GGGCTGCTCC 2881 GAGCTTTCCC GGCACAGGGT CCTGAGGAGT CTGTGCTCCC TAGCAGCTGA AGGGATTCAG 2941 CACCAGAGGT TCCTATTTGA AGAAGCTGAG CTCAGGAAAC ATAATTTAGA TGGCCCCAGG 3001 CTTGCCGCTT TCCTGAGTAG TAACGACTAC CAATTGGGAC TTGCCATCAA GAAGTTCTAC 3061 AGCTTCCGCC ACATCAGCTT CCAGGACTTT TTTCATGCCA TGTCTTACCT GGTGAAAGAG 3121 GACCAAAGCC GGCTGGGGAA GGAGTCCCGC AGAGAAGTGC AAAGGCTGCT GGAGGTAAAG 3181 GAGCAGGAAG GGAATGATGA GATGACCCTC ACTATGCAGT TTTTACTGGA CATCTCGAAA 3241 AAAGACAGCT TCTCGAACTT GGAGCTCAAG TTCTGCTTCA GAATTTCTCC CTGTTTAGCG 3301 CAGGATCTGA AGCATTTTAA AGAACAGATG GAATCTATGA AGCACAACAG GACCTGGGAT 3361 TTGGAATTCT CCCTGTATGA AGCTAAAATA AAGAATCTGG TAAAAGGTAT TCAGATGAAC 3421 AATGTATCAT TCAAGATAAA ACATTCAAAT GAAAAGAAAT CACAGAGCCA GAATTTATTT 3481 TCTGTCAAAA GCAGCTTGAG TCATGGACCT AAGGAGGAGC AAAAATGTCC TTCTGTCCAT 3541 GGACAGAAGG AGGGCAAAGA TAATATAGCA GGAACACAAA AGGAAGCTTC TACTGGAAAA 3601 GGCAGAGGGA CAGAGGAAAC ACCAAAAAAT ACTTACATAT AAGGATCCTG ATATCCAGCA 3661 CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG 3721 CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA 3781 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT 3841 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA 3901 GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC 3961 AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 4021 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 4081 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 4141 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 4201 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 4261 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 4321 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 4381 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG 4441 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 4501 AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 4561 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA 4621 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG 4681 AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG 4741 GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG 4801 AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG 4861 CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG 4921 ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC 4981 TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA 5041 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC 5101 TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG 5161 TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT 5221 TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG 5281 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA 5341 GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT 5401 TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG 5461 GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG 5521 GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC 5581 GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT 5641 GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA 5701 TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG 5761 GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA 5821 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG 5881 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG 5941 CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 6001 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 6061 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 6121 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 6181 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 6241 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 6301 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 6361 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 6421 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 6481 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 6541 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 6601 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 6661 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 6721 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 6781 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 6841 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 6901 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 6961 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 7021 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 7081 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 7141 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 7201 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 7261 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 7321 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 7381 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 7441 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 7501 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 7561 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 7621 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 7681 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 7741 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 7801 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 7861 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 7921 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 7981 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 8041 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 8101 AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.