pLV3-CMV-KCNG1-3×FLAG-CopGFP-P

pLV3-CMV-KCNG1-3×FLAG-CopGFP-Puro Plasmid

PVT112179

pLV3-CMV-KCNG1-3×FLAG-CopGFP-Puro Plasmid Information

Product Name: pLV3-CMV-KCNG1(human)-3×FLAG-CopGFP-Puro
Alertnative name: NM_002237.4;K13; KCNG; kH2; KV6.1
Gene lenght: 1539
Host: mammalian cells,lentivirus
Use(s): Gene expession
Fragment type: ORF
Species: Human
Resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Competent cells: Stbl3
Growth Temperature: 37℃
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KCNG1-3×FLAG-CopGFP-Puro Plasmid Description

pLV3-CMV-KCNG1(human)-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-KCNG1-3×FLAG-CopGFP-Puro Plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga ccctcttacc gggagacaat 2341 tctgactacg actacagcgc gctgagctgc acctcggacg cctccttcca cccggccttc 2401 ctcccgcagc gccaggccat caagggcgcg ttctaccgcc gggcgcagcg gctgcggccg 2461 caggatgagc cccgccaggg ctgtcagccc gaggaccgcc gccgtcggat catcatcaac 2521 gtaggcggca tcaagtactc gctgccctgg accacgctgg acgagttccc gctgacgcgc 2581 ctgggccagc tcaaggcctg caccaacttc gacgacatcc tcaacgtgtg cgatgactac 2641 gacgtcacct gcaacgagtt cttcttcgac cgcaacccgg gggccttcgg cactatcctg 2701 accttcctgc gcgcgggcaa gctgcggctg ctgcgcgaga tgtgcgcgct gtccttccag 2761 gaggagctgc tgtactgggg catcgcggag gaccacctgg acggctgctg caagcgccgc 2821 tacctgcaga agattgagga gttcgcggag atggtggagc gggaggaaga ggacgacgcg 2881 ctggacagcg agggccgcga cagcgagggc ccggccgagg gcgagggccg cctggggcgc 2941 tgcatgcggc gactgcgcga catggtggag aggccgcact cggggctgcc tggcaaggtg 3001 ttcgcctgcc tgtcggtgct cttcgtgacc gtcaccgccg tcaacctctc cgtcagcacc 3061 ttgcccagcc tgagggagga ggaggagcag ggccactgtt cccagatgtg ccacaacgtc 3121 ttcatcgtgg agtcggtgtg cgtgggctgg ttctccctgg agttcctcct gcggctcatt 3181 caggcgccca gcaagttcgc cttcctgcgg agcccgctga cgctgatcga cctggtggcc 3241 atcctgccct actacatcac gctgctggtg gacggcgccg ccgcaggccg tcgcaagccc 3301 ggcgcgggca acagctacct ggacaaggtg gggctggtgc tgcgcgtgct gcgggcgctg 3361 cgcatcctgt acgtgatgcg cctggcgcgc cactccctgg ggctgcagac gctggggctc 3421 acggcccgcc gctgcacccg cgagttcggg ctcctgctgc tcttcctctg cgtggccatc 3481 gccctcttcg cgcccctgct ctacgtcatc gagaacgaga tggccgacag ccccgagttc 3541 accagcatcc ctgcctgcta ctggtgggct gtcatcacca tgacgacggt gggctatggc 3601 gacatggtcc ccaggagcac cccgggccag gtagtggccc tgagcagcat cctgagcggc 3661 atcctgctca tggccttccc agtcacctcc atcttccaca ccttctcccg ctcctacctg 3721 gagctcaagc aggagcaaga gagggtgatg ttccggaggg cgcagttcct catcaaaacc 3781 aagtcgcagc tgagcgtgtc ccaggacagt gacatcttgt tcggaagtgc ctcctcggac 3841 accagagaca ataacggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 3901 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 3961 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 4021 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 4081 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 4141 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 4201 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 4261 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4321 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4381 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4441 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 4501 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 4561 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 4621 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 4681 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 4741 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 4801 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 4861 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 4921 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 4981 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 5041 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 5101 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 5161 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 5221 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 5281 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 5341 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 5401 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5461 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 5521 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 5581 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5641 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5701 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5761 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5821 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 5881 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 5941 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 6001 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 6061 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6121 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6181 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6241 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6301 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6361 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6421 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 6481 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 6541 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 6601 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 6661 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 6721 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 6781 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 6841 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 6901 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 6961 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 7021 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 7081 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 7141 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 7201 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 7261 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 7321 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7381 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7441 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7501 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7681 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7741 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7801 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 7861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 7921 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 7981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 8041 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8101 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8221 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 8281 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8341 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8401 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8461 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8521 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8581 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8641 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8701 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8761 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 8821 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 8881 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 8941 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9001 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9061 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9121 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9181 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9361 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 9421 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 9481 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 9541 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 9601 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9661 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 9721 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 9781 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.