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文献和实验Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
Mag-Binds Plant DNA Kit Magnetic Fast Protocol
during the transfer in next step. 5. Carefully transfer 400 ul supernatant to 2ml Deep Well Plate, making sure not to disturb the pellet or transfer any debris. 6. Add 20 ul MagSi Particles and 280 ul absolute thanol to each well. Shaking to mix for 2 min
Mag-Binds Plant DNA Kit Magnetic 96 Protocol
may float. Be careful to avoid those particles during the transfer in next step. 4. Carefully transfer 400 ul supernatant to 2ml Deep Well Plate, making sure not to disturb the pellet or transfer any debris. 5. Add 20 ul MagSi Particles and 280
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