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文献和实验Tissue Factor/VIIa in Thrombosis and Cancer
Tissue factor (TF) is a transmembrane glycoprotein that functions as a receptor and cofactor for activated factor VII (factor VIIa) to initiate blood coagulation. Although TF has been characterized best for its role in blood coagulation
Regulation of Krpple-Like Factor 5 by Targeted Protein Degradation
whether a protein is ubiquitinated have been described [Kaiser, Tagwerker (Methods Enzymol 399:243–248, 2005); Bloom, Pagano (Methods Enzymol 399:249–266, 2005)]. This chapter focuses on experimental approaches testing KLF5 transcription factor ubiquitination
Factor Xa Cleavage of MBP-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of factor Xa, temperature and length of incubation must be calibrated
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