• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Regulation of Krpple-Like Factor 5 by Targeted Protein Degradation

        互联网

        881
        Transcription factors are usually unstable proteins. The degradation of the majority of transcription factors is through the ubiquitin proteasome pathway and is tightly regulated by E3 ubiquitin ligases. KLF5 is an important transcription factor regulating cell proliferation, cell cycle, survival, migration, differentiation, angiogenesis, and stem cell self-renewal. We have shown that the WWP1 E3 ligase targets KLF5 for ubiquitin-mediated degradation. Several methods to determine whether a protein is ubiquitinated have been described [Kaiser, Tagwerker (Methods Enzymol 399:243–248, 2005); Bloom, Pagano (Methods Enzymol 399:249–266, 2005)]. This chapter focuses on experimental approaches testing KLF5 transcription factor ubiquitination and degradation by its E3s.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序