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硝基化α-突触核蛋白图片

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  • 询价
  • 上海博湖
  • BH-K20224
  • 进口、国产
  • 2026年03月06日
  • WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
  • 详见说明书
  • 详见说明书
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      硝基化α-突触核蛋白图片

    • 抗体英文名

      Anti-nitrated Alpha-synuclein (nitro-Tyr39)

    • 靶点

      详见说明书

    • 浓度

      1mg/1ml

    • 应用范围

      WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

    • 宿主

      详见说明书

    • 供应商

      上海博湖

    • 库存

      大量

    • 级别

      详见说明书

    • 目录编号

      详见说明书

    • 抗原来源

      Rabbit

    • 保质期

      详见说明书

    • 适应物种

      详见说明书

    • 标记物

      详见说明书

    • 克隆性

      多克隆

    • 保存条件

      Store at -20 °C

    • 形态

      详见说明书

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised nitrylpeptide derived from human Alpha-synuclein around the nitrosation site of Tyr39

    • 规格

      0.2ml/200μg

    英文名称  Anti-nitrated Alpha-synuclein (nitro-Tyr39)
    中文名称 硝基化α-突触核蛋白图片 
         SYUA_HUMAN; nitrated Alpha-synuclein (nitrated-Tyr39); nitrated Alpha-synuclein (nitro-Tyr39); nitrated Alpha synuclein; nitrated Alpha-Synuclein; Alpha synuclein; Alpha-synuclein, isoform NACP140; alphaSYN; MGC105443; MGC110988; MGC127560; MGC64356; NACP; Non A beta component of AD amyloid; Non A4 component of amyloid precursor; Non-A-beta component of alzheimers disease amyloid , precursor of; PARK 1; PARK 4; PARK1; PARK4; Parkinson disease familial 1; PD 1; PD1; SNCA; Snca synuclein, alpha (non A4 component of amyloid precursor); Synuclein alpha; Synuclein, alpha (non A4 component of amyloid precursor). 

    产品细节图片1
    硝基化α-突触核蛋白图片      1mg/1ml
     0.1ml/200μg
    抗体来源  Rabbit
    克隆类型   polyclonal
    交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig
    产品类型    一抗  
    研究领域    细胞生物 神经生物学 细胞凋亡
    蛋白分子量  predicted molecular weight: 15kDa
         Lyophilized or Liquid
      KLH conjugated Synthesised nitrylpeptide derived from human Alpha-synuclein around the nitrosation site of Tyr39
         IgG
    纯化方法   affinity purified by Protein A
      Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

    产品细节图片2
    硝基化α-突触核蛋白图片产品应用  WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
    (石蜡切片需做抗原修复) 
     not yet tested in other applications.
     optimal dilutions/concentrations should be determined by the end user.  
    保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
    Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
    产品介绍 The synuclein family members, including Alpha-synuclein (also designated NACP for non-Beta-Amyloid component) and Beta-synuclein, are predominantly expressed in the brain and are speculated to be involved in synaptic regulation and neuronal plasticity. Alpha-synuclein is localized to neuronal cell bodies and synapses. Alpha-synuclein was first identified as a component of Alzheimer’s disease amyloid plaques. Abnormal platelet function in Alzheimer’s disease has been demonstrated. During megakaryocytic differentiation Alpha-synuclein has been found to be upregulated, while Beta-synuclein is downregulated, indicating that coordinate expression of synucleins may be important during hematopoetic cell differentiation. A mutant form of Alpha-synuclein has been found in patients with early onset Parkinson’s disease.

    Function : May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.Subunit : Soluble monomer which can form filamentous aggregates. Interacts with UCHL1. Interacts with phospholipase D and histones.Subcellular Location : Cytoplasm. Membrane. Nucleus. Cell junction, synapse. Note=Membrane-bound in dopaminergic neurons.Tissue Specificity : Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.Post-translational modifications : Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.Ubiquitinated. The predominant conjugate is the diubiquitinated form.DISEASE : Note=Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.Defects in SNCA are the cause of Parkinson disease type 1 (PARK1) [MIM:168601]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. Additional features are characteristic postural abnormalities, dysautonomia, dystonic cramps, and dementia. The pathology of Parkinson disease involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. The disease is progressive and usually manifests after the age of 50 years, although early-onset cases (before 50 years) are known. The majority of the cases are sporadic suggesting a multifactorial etiology based on environmental and genetic factors. However, some patients present with a positive family history for the disease. Familial forms of the disease usually begin at earlier ages and are associated with atypical clinical features.Defects in SNCA are the cause of Parkinson disease type 4 (PARK4) [MIM:605543]. A complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.Defects in SNCA are the cause of dementia Lewy body (DLB) [MIM:127750]. A neurodegenerative disorder clinically characterized by mental impairment leading to dementia, parkinsonism, often with fluctuating cognitive function, visual hallucinations, falls, syncopal episodes, and sensitivity to neuroleptic medication. Brainstem or cortical intraneuronal accumulations of aggregated proteins (Lewy bodies) are the only essential pathologic features. Patients may also have hippocampal and neocortical senile plaques, sometimes in sufficient number to fulfill the diagnostic criteria for Alzheimer disease.Similarity : Belongs to the synuclein family.
    产品细节图片3
    硝基化α-突触核蛋白图片抗体的鉴定:
    1)抗体的效价鉴定:不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
    2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 
    如果所用抗原浓度IC50浓度为pg/,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
    3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
    种属:
    兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml 抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体 抗体的交叉反应: 人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,ElisaWB,免疫荧光等实验。
    硝基化α-突触核蛋白图片实验原理 :
    1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
    2)活补体:IgMIgG1IgG2IgG3可通过经典途径激活补体,凝聚的IgAIgG4IgE可通过替代途径激活补体。
    3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
    4)可通过胎盘及粘膜:免疫球蛋白GIgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
    免疫。免疫球蛋白AIgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
    5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
    6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,6070即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。

    产品细节图片4
    VDAC1 抗体, 兔多抗, 抗原亲和纯化 WB   IHC-P    50 µg
    Beta-Tubulin Loading Control 抗体, 鼠单抗 WB   IF   ICC/IF   50 µL
    Beta-Actin 抗体, 兔多抗, 抗原亲和纯化 WB   IHC-P   IP   50 µg
    pCMV3-C-His 阴性对照载体 (CHis标签
    pCMV3-N-Myc 阴性对照载体 (NMyc标签)
    LDLR / LDL Receptor 抗體, 兔多抗, 抗原親和純化 ELISA    50 µg

    LDLR / LDL Receptor 抗體, 兔多抗 ELISA    400 µg
    EMR1 抗體, 兔多抗, 抗原親和純化 WB    50 µg
    LDLR / LDL Receptor 抗體, 兔單抗 ELISA    50 µg
    LDL Receptor / LDLR 抗體, 兔單抗 FCM    50 µg
    Immunoprecipitation/ IP Kit - Anti-Angiopoietin-2 / ANG2 Immunomagnetic Beads IP    5 mL

    Immunoprecipitation/ IP Kit - Anti-USH1C / Harmonin Immunomagnetic Beads IP    5 mL
    Immunoprecipitation/ IP Kit - Anti-HtrA2 Immunomagnetic Beads IP    5 mL
    Immunoprecipitation/ IP Kit - Anti-CDK2 Immunomagnetic Beads IP    5 mL
    Immunoprecipitation/ IP Kit - Anti-Aminoacylase-1 / ACY1 Immunomagnetic Beads IP    5 mL
    血清肝胆酸(cholyglycine)ELISA试剂盒 15.6-1000 pg/mL

    ELISA Kit for cholyglycine 15.6-1000 pg/mL
    人酶(Carbonic anhydrase 12)ELISA试剂盒 0.156-10 ng/mL
    ELISA Kit for Human Carbonic anhydrase 12 0.156-10 ng/mL
    人含CASP8FADD样凋亡调节因子(CFLAR)ELISA试剂盒 15.6-1000 pg/mL
    人犬尿氨酸/线粒体氨基已二酸转氨酶α(AADAT)ELISA试剂盒 0.078-5 ng/mL

    ELISA Kit for Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial 0.078-5 ng/mL
    人氨肽酶O(AP-O)ELISA试剂盒 0.312-20 ng/mL
    ELISA Kit for Human Aminopeptidase O 0.312-20 ng/mL
    AP-1复合体亚基mu-1(AP-1 complex subunit mu-1)ELISA试剂盒 0.156-10 ng/mL
    硝基化α-突触核蛋白图片P选择素(P-Selectin/CD62P/GMP140)ELISA试剂盒 62.5-4000 pg/mL

    ELISA Kit for Human P-selectin 62.5-4000 pg/mL
    L选择素(L-Selectin/CD62L)ELISA试剂盒 0.78-50 ng/ml
    ELISA Kit for Human L-selectin 0.78-50 ng/ml
    E选择素(E-Selectin/CD62E)ELISA试剂盒 0.156-10 ng/mL
     

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    图标文献和实验
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    • α 突触核蛋白简介

      存在于突触前末梢和神经元核内的蛋白质,有 α、β 和 γ 等类型。α 突触核蛋白与帕金森病等疾病有关。

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    • α-突触核蛋白(a-Synuclein)ELISA试剂盒 说明书

        上海西唐生物科技有限公司   021-55229872,  65333639   www.westang.com 人 α- 突触核蛋白 ( a -Synuclein)ELISA 试剂盒   ( 用于血清、血浆、细胞培养上清液和其它生物体液内 )   原理 本实验采用双抗体夹心  ABC-ELISA 法。用抗人   a -Synuclein   单抗包被于酶标板上,标准品和样品中的  

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