Recombinant protein of human K

Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets

. nos. 1830‐9610) 0.65‐µm pore hydrophilic low‐protein‐binding filter plates (Millipore) Glutathione Sepharose beads (e.g

Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions

a titration of protein A microbeads (as indicated) against a constant amount of Fc‐fusion protein (4 µg). The minimal bead concentration where no free Fc‐fusion remains is selected as optimal (40 µl, the black curve in this case). This figure is reproduced

Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)

Buffer E 20 mM HEPES-KOH pH 7.8 (1 M stock) 5 mM Potassium Acetate (1 M stock) 0.5 mM MgCl2   (1 M stock) 0.5 mM DTT (1 M stock) Inhibitors: 10 µg