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- 文献和实验
- 技术资料
- 英文名:
Recombinant protein of human lysyl oxidase (LOX), 20 µg
- 供应商:
OriGene
- 规格:
20 ug
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
according to the method of Laemmli (6 ) on a polyacrylamide-gradient (8-16%) slab gel (Tefco, Tokyo) with the electrophoresis buffer (25 mM Tris, 192 mM glycine, and 0.1% SDS) at 20 mA for about 1.5 hr. Hog kidney D-amino-acid oxidase (10 ng, Sigma) and prestained protein
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
. nos. 1830‐9610) 0.65‐µm pore hydrophilic low‐protein‐binding filter plates (Millipore) Glutathione Sepharose beads (e.g
Methods for the Detection of D-Amino-Acid Oxidase
al. ( 5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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