- ￥2900
- OriGene
- TP313132
- 2026年09月14日
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- 文献和实验
- 技术资料
- 英文名：
Recombinant protein of human hydroxysteroid (17-beta) dehydrogenase 13 (HSD17B13), transcript variant A, 20 µg
- 供应商：
OriGene
- 规格：
20 ug
一、产品数据
HEK293表达重组人源HSD17B13活性蛋白
Recombinant protein of human hydroxysteroid (17-beta) dehydrogenase 13 (HSD17B13), transcript variant A, 20 µg
| Description | Recombinant protein of human hydroxysteroid (17-beta) dehydrogenase 13 (HSD17B13), transcript variant A, 20 µg |
| Species | Human |
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence |
Protein Sequence (show) 见官网
|
| Tag | C-Myc/DDK |
| Predicted MW | 33.5 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage Condition | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
二、活性验证数据
HSD17B13 activity was determined by measuring NADH production using an MTS-based colorimetric assay. Reactions were performed at 37 °C for 1 h in 100 µL containing 20 mM phosphate buffer (pH 7.3), 150 mM NaCl, 0.02% BSA, 0.002% Tween-20, 0.5 mM NAD⁺, 75 µM estradiol, and varying amounts of purified HSD17B13. Reactions were terminated by NaOH treatment followed by acid neutralization and centrifugation. Supernatants were subjected to a coupled reaction with alcohol dehydrogenase and ethanol, and NADH levels were quantified using MTS reagent with absorbance measured at 492 nm after incubation at 37 °C. NADH standards were processed in parallel to generate a calibration curve for quantification.
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文献和实验[1]Discovery of a Novel Potent and Selective HSD17B13 Inhibitor, BI-3231, a Well-Characterized Chemical Probe Available for Open Science ,null, Journal of Medicinal Chemistry ,PubMed ID 36727857 [HSD17B13]
[2]Genome-wide association study of non-alcoholic fatty liver and steatohepatitis in a histologically-characterised cohort ,Anstee, QM;Darlay, R;Cockell, S;Meroni, M;Govaere, O;Tiniakos, D;Burt, AD;Bedossa, P;Palmer, J;Liu, YL;Aithal, GP;Allison, M;Yki-Järvinen, H;Vacca, M;Dufour, JF;Invernizzi, P;Prati, D;Ekstedt, M;Kechagias, S;Francque, S;Petta, S;Bugianesi, E;Clement, K;Ratziu, V;Schattenberg, JM;Valenti, L;Day, CP;Cordell, HJ;Daly, AK;, ;, J. Hepatol. ,PubMed ID 32298765 [HSD17B13]
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