Recombinant protein of human h

Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets

. nos. 1830‐9610) 0.65‐µm pore hydrophilic low‐protein‐binding filter plates (Millipore) Glutathione Sepharose beads (e.g

Detection of Protein‐Protein Interactions by Coprecipitation

of interest is precipitated, and the precipitate is tested for a second specifically associated protein. The approach can be used for native or epitope?tagged proteins for which antibodies are available, or for recombinant proteins that bind with high affinity to a molecule

Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions

a titration of protein A microbeads (as indicated) against a constant amount of Fc‐fusion protein (4 µg). The minimal bead concentration where no free Fc‐fusion remains is selected as optimal (40 µl, the black curve in this case). This figure is reproduced