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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
WB
- 适应物种:
Human
- 标记物:
Unconjugated
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 形态:
Liquid
- 规格:
50μl/100μl
| 规格: | 50μl | 产品价格: | ¥1024.0 |
|---|---|---|---|
| 规格: | 100μl | 产品价格: | ¥1824.0 |
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
INTRODUCTION The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae
Purifying Protein from Inclusion Bodies
14 until no more of the pellet dissolves. 16.Dialyze 8 M urea solutions in 4 L of a 50 mM tris-HCl buffer solution at pH 8.5 using 3.5 kD molecular weight cutoff dialysis tubing for 2 days.Repeat,dumping out buffer and replacing with fresh buffer
Protein Purification HD exon 1, 16, 38, 56, and 82 CAGs fused with GST. Making constructs. Exon I of HD gene was amplified from partial cDNA constructs obtained from normal (16 CAGs) and patient (48 CAGs). The primers
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