- ￥1024 - 1824
- 正能
- 125501
- 中国
- 2026年06月01日
- WB,IHC-P
- Human
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- 文献和实验
- 技术资料
- 应用范围：
WB,IHC-P
- 适应物种：
Human
- 标记物：
Unconjugated
- 保存条件：
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 形态：
Liquid
- 规格：
50μl/100μl
| 规格： | 50μl | 产品价格： | ￥1024.0 |
|---|---|---|---|
| 规格： | 100μl | 产品价格： | ￥1824.0 |
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
INTRODUCTION The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae
. When the HD gene was cloned in 1993, it was discovered that the causative mutation was an expansion of a CAG trinucleotide repeat (1 ) and to date HD is one of 14 trinucleotide repeat diseases (2 ). The expanded CAG sequence in exon 1 of the HD gene, IT15
Purifying Protein from Inclusion Bodies
.Save supernatant.The pellet remaining will be the inclusion bodies. 14.Add 20 ml of 8 mM urea (add DTT to 8 mM urea if purifying proteins with cysteine residues)and dissolve the pellet.Heating in a bath (37-50℃)may facilitate the pellet dissolving. 15.Repeat step
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