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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
KLH-conjugated synthetic peptide: EQKLISEEDL (Myc tag), derived from UniProt: Q6LBK7
- 形态:
Antigen affinity purified serum, in PBS pH 7.4
- 保存条件:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the c
- 克隆性:
Polyclonal
- 适应物种:
Myc epitope tag, fused to N- or C-terminal of proteins
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
Immunofluorescence (IF)
- 靶点:
Anti-Myc tag (rabbit antibody, polyclonal)
- 抗体英文名:
Anti-Myc tag (rabbit antibody, polyclonal)
- 抗体名:
Anti-Myc tag (rabbit antibody, polyclonal)
- 规格:
50 µg
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文献和实验干货 | CUT and Tag 让 ChIP-Seq更简单高效!
说明书推荐的反应体系进行 CUT&Tag 实验;其中,pG-Tn5 转座子使用的终浓度为 0.04μM ,一抗为 H3K27me3(CST,#9733),二抗为 Goat anti Rabbit(Bioworld,#BS13271)。 A、文库峰型检测 使用 Agilent 2100 Bioanalyzer 进行文库分布检测。 图 2. 使用 TD901 构建 100 cells CUT&Tag 文库峰型图 图 3. 使用 TD901 构建 10,000 cells CUT&Tag
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Anti-DYKDDDDK tag (L5) Affinity Gel
or 0.22µm filter to prepare for L5 purification. Part II. Resin Preparation 1. Thoroughly suspend the resin by gentle inversion until the bottle of Anti- DYKDDDDK-tag (L5) affinity gel is a uniform suspension of gel beads. Transfer
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