NEBNext 免疫测序试剂盒 - 小鼠 | NEB

品牌	货号	产品中文名称	产品英文名称
NEB	E6330	NEBNext 免疫测序试剂盒 - 小鼠	NEBNext®Immune Sequencing Kit (Mouse)

货号	产品中文名称	产品英文名称	规格
E6330S	NEBNext 免疫测序试剂盒 - 小鼠	NEBNext® Immune Sequencing Kit (Mouse)	24 rxns

产品特点

使用 NEBNext^® 免疫测序试剂盒（小鼠）可对 B 细胞和 T 细胞免疫组库进行全长测序。提高测序准确性后，可分析所有相关背景下的体细胞突变（如：V、D、J 区域以及 IgM、IgD、IgG、IgA 和 IgE 亚类）。分析 BCR 轻链、BCR 重链、TCRα、TCRβ、TCRγ 和 TCRδ 链。该试剂盒提供唯一分子标签序列（UMI），用于对起始分子的鉴别。

• 通过对受体序列的深入分析，揭示复杂的免疫系统
• 对 B 细胞受体（BCR）和 T 细胞受体（TCR）进行富集和测序
• 制备 B 细胞和 T 细胞的全长免疫组库
• 使用唯一分子标签序列（UMI）准确定量转录本
• 使用基于开源软件 pRESTO 工具包的生物信息流程分析数据（教程）

注意：我们提供用于人类样本免疫组库测序的单独试剂盒。

The NEBNext Immune Sequencing Kit (Mouse) enables exhaustive profiling of somatic mutations in the full-length immune gene repertoires of B cells and T cells, via the expression of complete antibody chains. This includes modular primer sets, providing information for complete V, D, and J segments and full isotype information analysis (IgM, IgD, IgG, IgA and IgE), and TCRα, TCRβ, TCRγ and TCRδ chain characterization. A unique, UMI-based mRNA barcoding process allows PCR copies derived from an individual molecule to be converted to a consensus sequence. This improves sequence accuracy and eliminates PCR bias. Based on the open-source pRESTO toolkit, a workflow is available via the Galaxy platform to enable robust bioinformatic analysis locally or in a cloud environment. A workflow tutorial ensures that users who are not familiar with Galaxy can successfully use the analysis workflow,
Immune repertoire sequencing is frequently used to analyze immune responses, both current and distant. Areas of particular interest include characterization of autoimmune diseases, oncology, discovery of neutralizing antibodies against infectious disease, tumor-infiltrating lymphocytes and use as a tool to study residual disease. Recent improvements in read lengths and throughputs of next-generation sequencing (NGS) platforms have resulted in a rise in the popularity of immune repertoire sequencing.
Defining features of the NEBNext Immune Sequencing method include:

• Generation of full-length variable sequences (including isotype information), allowing downstream antibody synthesis and functional characterization not possible with approaches sequencing only the CDR3 region
• Eliminated use of variable region primers, reducing primer pool complexity and realizing unbiased and simultaneous recovery of B cell and T cell receptor transcripts. 
• Minimized PCR bias and improved sequencing accuracy by allowing a consensus to be generated from duplicate sequencing reads originating from the same transcript; UMIs enable accurate quantitation of each clone present in the sample. 
• Optimized high target-capture efficiency for immune repertoire sequencing and analysis from sub-microgram quantities of total RNA.

备注：以上仅为 E6330 的部分产品信息，如需NEB货号 E6330 的完整产品信息及相关的实验操作手册等等，请通过NEB官网查找对应的货号获取。