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- NE135/Bio
- 2026年02月22日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京柏瑞图科技有限公司
- 规格：
NE135/Bio-1 ml
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining
: Indirect immunohistochemistry with avidin-biotin peroxidase(e.g rabbit anti antigen X, goat anti-rabbit biotin, avidin HRP) 1- Wash twice in TBS 0.05M pH7.5, to which 0.01% Tween 20 has been added. 2- Briefly blot the slides without letting
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