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文献和实验Pulse-Chase mRNA decay assay in HeLa Tet-off cells:
This protocol is for four time-points in 12-well plates. Scale up or down as needed. Day 1: Plate cells: Split one or more 10-cm plate(s) with 80-100% confluent HeLa Tet-off cells to a final volume of 10-11 ml. Make sure
Single Strand DNA Prep. for Sequencing
This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly). Solutions 2X YT Media 16 g tryptone 10 g yeast extract 5 g NaCl 1 ml 1N NaOH up to 1 liter with Q Ampicillin Stock (1000X) 0.15 g
> B. DNA PREPS FROM MANY INDIVIDUAL FLIES A similar method can be used with 96-well (8 x 12) micro plates to prepare DNA from a large number of individual flies. Up to 80 flies can be tested with a single plate. Place one anesthetized
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