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- Norgen Biotek
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- 2026年02月19日
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北京柏瑞图科技有限公司
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文献和实验Quantitation of Liver-Stage Parasites by Automated TaqMan Real-Time PCR
The liver stages of the malaria parasite have long been a difficult part of the life cycle to study. Very little is known about the localization of the parasite within the liver and no method for the estimation of liver parasite burden
TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
in this unit describes the steps for gene expression analysis of Haemophilus influenzae in broth culture. Steps for hot acid phenol RNA extraction, RNA cleanup, quantitation, assessment, and preparation for TaqMan qRT?PCR are described. The validation, setup
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