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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
1 mL (100 ug/ml)
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Elabscience | BV2细胞M1极化造模与流式检测,这份Protocol请收好
Buffer(E-CK-A107)或PBS缓冲液重悬细胞沉淀,调整细胞密度为1-10×106个/mL; (3)取100 μL细胞悬液,每组加入2 μL Purified Anti-Mouse CD16/32 Antibody(E-AB-F0997A),轻轻吹打混匀,室温避光孵育10 min封闭细胞表面的Fc受体; (4)加入各流式抗体(5 μL/test),轻轻吹打混匀,4℃避光孵育30 min; (5)孵育完成后,每管加入1 mL Cell Staining Buffer(E-CK-A107)轻轻
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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