- 询价
- Enzo Life Sciences
- USA
- ADI-SPA-781-E
- 2026年03月10日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
Neobioscience
- 保存条件：
-20
- 规格：
1 x 100.0 x µg
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文献和实验The production of monoclonal antibody by hybridoma
{Cell Mab (BD), 10% FBS (or HS) ; 5% Origen HCF (hybridoma cloning factor) containing 4mM L-glutamine and antibiotics} to be used for plating hybridomas after the fusion. Hybridoma medium CM - HT (NO AMINOPTERIN) {Cell Mab
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
Intracellular Cytokine Staining Protocol
to produce IFN-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used
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