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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
100 ug
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
the bead pellet by adding 0.5 mL of buffer to each sample tube. 11) Add one drop of negative beads (Component B in the AbC™ Anti-Mouse Bead Kit) to sample tube(s) containing the AbC™ capture beads. 12) Add one drop of ArC™ negative beads
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
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