细胞凋亡和细胞坏死检测试剂盒

Cell Meter细胞凋亡和细胞坏死检测试剂盒 *三色* -Cell Meter Apoptotic and Necrotic Detection Kit *Tricolor
英文名称：Cell Meter Apoptotic and Necrotic Detection Kit *Tricolor

Cell Meter检测试剂盒是一类用于检测细胞功能的系列工具，包括细胞活性、细胞毒性、细胞凋亡、细胞膜电位以及细胞周期等方面的指标。每种检测方案均能提供不同荧光颜色的检测方案。这些高效的检测方案为从多角度研究细胞功能活动提供了一种十分有效的方法。

Cell Meter细胞凋亡和细胞坏死检测试剂盒 *三色* 为检测细胞凋亡、病理或者正常细胞而开发设计的。凋亡被概括被为激活的并避免诱导炎症的、自性发细胞程序化分解。在凋亡细胞中，PS从胞内移位到胞外表 面，因此PS出现在细胞的外表面可以作为细胞凋亡的起始或者中间阶段的通用指示器，也可以通过其形态学改变来测定。该试剂盒中PS指示器结合膜上的PS 后，产生绿色荧光(Ex/Em = 490/525 nm)。坏死则是以被动性和偶然性为特点的细胞死亡，起因于不受控制的外界环境改变，并导致炎症因子的释放。在晚期凋亡和坏死中，通过无膜渗透能力的深红 色荧光染料7-AAD (Ex/Em = 546/647 nm)，可以标记细胞核（因为这些细胞的细胞膜是不完整的）。此外，这个试剂盒也提供活细胞蓝色荧光染料Cyto Calcein Violet 450 (Ex/Em = 405/450 nm)来标记活细胞的细胞质。本试剂盒通过流式和荧光显微镜能最佳检测细胞凋亡（绿色），坏死（红 和/或者 绿色）以及正常细胞（蓝色）。
Our Cell Meter� assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used. This particular kit is designed to monitor cell apoptotic, necrotic and healthy cells. Apoptosis is described as an active, programmed process of autonomous cellular dismantling that avoids eliciting inflammation. In apoptosis, phosphatidylserine (PS) is transferred to the outer leaflet of the plasma membrane. As a universal indicator of the initial/intermediate stages of cell apoptosis, the appearance of phosphatidylserine on the cell surface can be detected before morphological changes are observed. The PS sensor used in this kit has green fluorescence (Ex/Em = 490/525 nm) upon binding to membrane PS. Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents. Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable 7-AAD (Ex/Em = 546/647 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis. In addition, this kit also provides a live cell cytoplasm labeling dye CytoCalcein� Violet 450 (Ex/Em = 405/450 nm) for labeling living cell cytoplasm. This kit is optimized to detect cell apoptosis (green), necrosis (green and/or red) and healthy cells (blue) with a flow cytometer and fluorescence microscope.