Recombinant Human Granulin, His-tagged
GRN-113H
Product Overview:
| |
Recombinant Human granulin encoding the human Pro-Granulins(Thr18-Leu593) was expressed inE.coli. It was recombined with a Hexa-his sequence of pET-28a vector at the N-Terminusand purified by Ni-NTA column. MW= 63.4 KD. |
| Description: |
Granulin is a protein that in humans is encoded by the GRN gene.Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. |
| Sequence: |
Human Pro-Granulins (Thr18-Leu593)18 TRCPDGQFCP VACCLDPGGA SYSCCRPLLD KWPTTLSRHL GGPCQVDAHC SAGHSCIFTV SGTSSCCPFP EAVACGDGHH CCPRGFHCSA DGRSCFQRSG NNSVGAIQCP DSQFECPDFS TCCVMVDGSW GCCPMPQASC CEDRVHCCPH GAFCDLVHTR CITPTGTHPL AKKLPAQRTN RAVALSSSVM CPDARSRCPD GSTCCELPSG KYGCCPMPNA TCCSDHLHCC PQDTVCDLIQ SKCLSKENAT TDLLTKLPAH TVGDVKCDME VSCPDGYTCC RLQSGAWGCC PFTQAVCCED HIHCCPAGFT CDTQKGTCEQ GPHQVPWMEK APAHLSLPDP QALKRDVPCD NVSSCPSSDT CCQLTSGEWG CCPIPEAVCC SDHQHCCPQG YTCVAEGQCQ RGSEIVAGLE KMPARRASLS HPRDIGCDQH TSCPVGQTCC PSLGGSWACC QLPHAVCCED RQHCCPAGYT CNVKARSCEK EVVSAQPATF LARSPHVGVK DVECGEGHFC HDNQTCCRDN RQGWACCPYR QGVCCADRRH CCPAGFRCAA RGTKCLRREA PRWDAPLRDP ALRQLL 593 |
| Source: |
E.coli. |
| Formulation: |
Lyophilized 100μg human GRN in 50μl of TBS (20mM Tris, 50mM NaCl, pH8.0). Carry free. |
| Purity: |
>95% by SDS gel. |
| Application: |
Cell biology. |
| Reconstitution: |
Add 500μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4℃, mix well. |
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Synonyms
GRN, PGRN, granulin, Acrogranin, propithelin, PC cell derived growth Factor, GEP, GP88, PEPI, PCDGF.
Introduction
A 88-kDa progranulin, also called proepithelin and PC cell-derived growth factor, is a single precursor protein of granulins which are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. Granulins are a variety of active, 6 kDa peptides and named granulin A (epithelin 1), granulin B (epithelin 2), granulin C, etc. Both the peptides and intact progranulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
Description
Progranulin Human Recombinant fused to FLAG at C-terminus produced in HEK is a single, glycosylated, polypeptide chain containing 1-593 amino acids and having a molecular mass of 70kDa.
The Progranulin is purified by standard chromatographic techniques.
Source
HEK 293 cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The protein solution contains 1xPBS.
Purity
Greater than 95.0% as determined by SDS-PAGE.
