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- 库存：
60
- 供应商：
深圳子科生物科技有限公司
- 规格：
500ul
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| PCR试剂 | G008 | Taq DNA Polymerase | 5000u |
| G126 | Taq DNA Polymerase | 10000U | |
| G009 | Taq DNA Polymerase | 1000U(200 μl) | |
| G013 | 2X PCR Taq MasterMix | 5.0 ml (200 Rxns) | |
| G013-dye | 2X PCR Taq MasterMix with dye | 5.0 ml (200 Rxns) | |
| G472 | Safe-Green ; 2X PCR Taq MasterMix | 5.0 ml (200 Rxns) | |
| G012 | Taq Plus DNA Polymerase | 250 U (50 µl) | |
| G040 | Taq Plus DNA Polymerase | 1000U(200 µl) | |
| G014 | 2X PCR Taq Plus MasterMix | 5.0 ml (200 Rxns) | |
| G014-dye | 2X PCR Taq Plus MasterMix with dye | 5.0 ml (200 Rxns) | |
| G277 | TaqFast DNA Polymerase | 250 U (50 µl) | |
| G278 | TaqFast DNA Polymerase | 1000U(200 μl) | |
| G280 | 2X PCR TaqFast MasterMix | ||
| G280-dye | 2X PCR TaqFast MasterMix with dye | ||
| G078 | Precision ; DNA Polymerase | 500 U (100 µl) | |
| G011 | HotStart DNA Polymerase | 250 U (50 µl) | |
| G039 | HotStart DNA Polymerase | 1000U(200 µl) | |
| G010 | dNTP Mix | 250µl | |
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文献和实验TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
Step 1: Take an 100 mL aliquot of frozen cells from the -80oC and innoculate about 500 ml to 1 L sterile LB broth. DO NOT ADD antibiotic
Preparation of Lactobacillus Competent Cells
Overview Instructions on how to prepare Lactobacillus plantarum competent cells before electrotransformation. Materials MRS media Culture of L. plantarum cells MgCL2 (10mM) sucrose glycerol
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