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| 品牌 | 货号 | 产品中文名称 | 产品英文名称 |
|---|---|---|---|
| NEB | R0140 | PstI | PstI |
| 货号 | 产品中文名称 | 产品英文名称 | 规格 |
|---|---|---|---|
| R0140L | PstI | PstI | 50,000 units |
| R0140M | PstI(高浓度) | PstI(High Concentration) | 50,000 units |
| R0140S | PstI | PstI | 10,000 units |
| R0140T | PstI(高浓度) | PstI(High Concentration) | 10,000 units |
| R0140V | PstI | PstI | 5,000 units |
产品特点
- 符合省时酶(Time-Saver™)标准,可在 5-15 分钟内完成酶切
- 可提供高保真(HF®)版本酶(NEB #R3140),随附 rCutSmart™ 缓冲液
- 随附 1 管 6X 紫色凝胶上样染料
- 限制性内切酶酶切位点:CTGCA/G
本酶提供高保真版 PstI-HF®(NEB #R3140)。
高保真(HF)限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性;统一缓冲液意味着更加直接、简化的样品处理过程。HF 内切酶还会显著降低星号活性。所有 HF 内切酶均符合省时酶(Time-Saver)标准,可在 5-15 分钟内酶切底物 DNA,也可实现过夜酶切而不会造成 DNA 降解。HF 限制性内切酶在开发时便将性能作为重要指标,可在更宽的条件范围下具有完全活性,最大限度地减少非特异性酶切产物,同时为实验设计提供灵活性。
产品来源
大肠杆菌菌株,其携带来自斯氏普罗威登斯菌(Providencia stuartii)164(ATCC 49762)的 PstI 基因。- 产品类别:
- P 至 R 字母开头的限制性内切酶产品,
- Time-Saver Qualified Restriction Enzymes
- 应用:
- BioBrick® Assembly,
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB
备注:以上仅为 R0140 的部分产品信息,如需NEB货号 R0140 的完整产品信息及相关的实验操作手册等等,请通过NEB官网查找对应的货号获取。
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文献和实验Determining the Direction of Replication Fork Movement
, with the enzyme of choice in various brands of agarose. The enzymes that cut to completion in Beckman LE agarose are AvaI, Bam HI, BglII, EcoRV, NcoI, PstI, PvuII, SacII, SnaBI, SpeI, StuI, XbaI and XhoI. (There are likely to be many others that also cut
genomic DNA digest-Molecular Biology
at the top of the gel, and hardly any smear at all. I have tried both NEB and Promega's enzymes, both new, with 500ng of DNA with 20 to 25 Units of enzyme for 3Hours+ or overnight. Does anyone know any issues related to those enzymes? or why one digest would work
of choice in various brands of agarose. The enzymes that cut to completion in Beckman LE agarose are AvaI, Bam HI, BglII, EcoRV, NcoI, PstI, PvuII, SacII, SnaBI, SpeI, StuI, XbaI and XhoI. (There are likely to be many others that also cut to completion
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