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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
Epredia
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1台
艾普迪Cytospin4涂片机提供了一种高效的方法,从液基中获取细胞用于显微镜检查⸺提高细胞产率并维持形态完整性。

优化细胞产率
- Cytospin4提供了一种快速、高效且经济的技术实现低细胞量样本的卓越细胞回收率,并高度保持细胞形态
- 保存了珍贵样本,用于进一步检测或细胞块制备
出色的细胞核显示效果
- 仪器采用离心原理,将细胞沉积到载玻片的固定区域,并将标本残夜用标本室的滤纸吸收
- 离心力使细胞呈扁平状,故细胞核的呈现效果极为清晰
- 仪器在快速旋转时,使离心漏斗标本室垂直倾斜,并使离心细胞位于载玻片的沉积区,从而形成单层细胞,使各细胞类型在离心过程中的呈现机会均等
安全性和易用性
- Cytospin4可在样本制备前、制备中、制备后保护操作者
- 包含一个高压灭菌密封头,可在微生物安全柜上装
载和卸载,以降低暴露于潜在病原体的几率 - 提供了一种极简技术,可在单次运行中集中处理多达12份样本
- 直观控制面板提供了简明的交互界面,用于输入时间、速度和加速度设置
- 带有可存储多达23个常用程序的受保护程序存储器

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文献和实验[1]Peng Y, Guan WJ, Tan KS, Zhu Z, Chen Z, Hong H, Wang Z, Tian T, Zi X, Ong YK et al: Aberrant localization of FOXJ1 correlates with the disease severity and comorbidities in patients with nasal polyps. Allergy Asthma Clin Immunol 2018, 14:71.
[2]Zi XX, Guan WJ, Peng Y, Tan KS, Liu J, He TT, Ong YK, Thong M, Shi L, Wang DY: An Integrated Analysis of Radial Spoke Head and Outer Dynein Arm Protein Defects and Ciliogenesis Abnormality in Nasal Polyps. Front Genet 2019, 10:1083.
[3]Miceska S, Stegel V, Bebar S, Kloboves-Prevodnik V: Cytopathological assessment is an accurate method for identifying immunophenotypic features and BRCA1/2 mutations of high-grade serous carcinoma from ascites. Cancer Cytopathol 2023, 131(3):188-197.
[4]Liu S, Cadaneanu RM, Zhang B, Huo L, Lai K, Li X, Galet C, Grogan TR, Elashoff D, Freedland SJ et al: Keratin 13 Is Enriched in Prostate Tubule-Initiating Cells and May Identify Primary Prostate Tumors that Metastasize to the Bone. PLoS One 2016, 11(10):e0163232.
[5]Piaton E, Faÿnel J, Hutin K, Ranchin MC, Cottier M. Conventional liquid-based techniques versus Cytyc Thinprep processing of urinary samples: a qualitative approach. BMC Clin Pathol. 2005 Oct 6;5:9. doi: 10.1186/1472-6890-5-9. PMID: 16207384; PMCID: PMC1260015.
[6]Gaber KA, Goldman JM, Farrell DJ. Cytological examination of the whole endo-bronchial brush in bronchoscopic diagnosis of lung cancer. Respir Med. 2002 Apr;96(4):259-61. doi: 10.1053/rmed.2001.1263. PMID: 12000005.
[7]Nakada K, Fujisawa K, Horiuchi H, Furusawa S. Studies on morphology and cytochemistry in blood cells of ayu Plecoglossus altivelis altivelis. J Vet Med Sci. 2014 May;76(5):693-704. doi: 10.1292/jvms.13-0584. Epub 2014 Jan 28. PMID: 24476851; PMCID: PMC4073338.
Preparation of cytospin from single cell suspension.
Cell number, speed and trivial details affect cytospin . Basic protocol: Prepare a cell suspension of not more than 0.5 x 106 cells / ml of protein-containing medium. Pre-label the slides. Procedure for old type
Female fertility is critically influenced by two events affecting oocytes during meiotic prophase progression: meiotic recombination between homologous chromosomes; and a major oocyte loss. It is technically challenging to examine
瑞氏(wright)染色法检测细胞凋亡 【材料与试剂】 Wright染液:称取Wright粉剂0.1g在碾钵内,充分碾磨,量取甲醇60ml,逐步加入,边加边碾磨,直到染料全部溶解,置试剂瓶中密封,1周后可用。 Wright磷酸盐缓冲稀释液:Wright染液10 ml,磷酸盐缓冲液20ml。 磷酸盐缓冲液:磷酸二氢钾6.63g,磷酸氢二钠2.56g,蒸馏水1000ml。 染色缸,离心涂片机,离心机,显微镜。 其它:甲醇,0.08











