SARS-CoV-2 Nucleocapsid Protei

Recombinant SARS-CoV-2 Nucleocapsid Protein V2

 

Catalog #:  DRA53       Derived From: E.coli   

 

Description:

Recombinant SARS-CoV-2 Nucleocapsid Protein is produced by our E.coli expression system and the target gene encoding Met1-Ala419 is expressed with a 6His tag at the N-terminus.

Accession:  QHD43423.2

Names(Known as): SARS-CoV-2 coronavirus NP Protein; SARS-CoV-2 np Protein; SARS-CoV-2 novel coronavirus Nucleoprotein Protein

 

Quality Control:

Mol Mass:  49.4kDa      AP Mol Mass:  50-60kDa, reducing conditions

Purity:  Greater than 95% as determined by reducing SDS-PAGE.

 

Formulation:

Supplied as a 0.2 μm filtered solution of 20mM HEPES, 100mM NaCl, pH 8.0.

 

Shipping:

The product is shipped on dry ice pack.Upon receipt, store it immediately at the temperature listed below.

 

Storage:

Reconstituted protein solution should be stored at ≤ -20°C.

 

Purification: 
Affinity purification chromatography.

 

 

Bioactivity-Elisa:

 

Immobilized SARS-CoV-2 NP Antibody(6G9)-Fc(Cat#DA027) at 5μg/ml(100 μl/well) can bind SARS-CoV-2 Nucleocapsid Protein-His(Cat#DRA53).The ED50 of SARS-CoV-2 Nucleocapsid Protein-His(Cat#DRA53) is 0.29 ug/ml.	000000	Immobilized SARS-CoV-2 NP Antibody(6G9)-Fc(Cat#DA027) at 5μg/ml(100 μl/well) can bind SARS-CoV-2 Nucleocapsid Protein-His(Cat#DRA53).The ED50 of SARS-CoV-2 Nucleocapsid Protein-His(Cat#DRA53) is 0.29 ug/ml.

 

 

Application:
Immunogen, calibrator or standard.

 

Background:

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may beinvolved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packagesthe positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role duringvirion assembly through its interactions with the viral genome and membrane protein M. Plays an important rolein enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of theconservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as adiagnostic tool.

 

FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY