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- 英文名：
Xa Removal Resin (2 x 5ml)
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文献和实验Factor Xa Cleavage of MBP-Fusion protein
temperature will increase cleavage; while lower incubation, less enzyme or lower temperature will decrease cleavage. As a general protocol you can use: 1. For 1ml of bed volume saturated resin, mix 50µg of Factor Xa from New England BioLabs solution in 1ml
into smaller pellets and processed more easily in their tubes. 4). Wash pellets with 200 mM sodium cacodylate buffer (3 x 5'). At this stage, the samples will be fixed so can be washed in any sutable buffer, and even water. Sodium cacodylate is suggested
Immunofluorescence on ultrathin resin sections
. Residual water is drained with filter paper along the outside of the loop. Note: air-dried resin sections can be stored for months prior to labelling. Procedure: Encircle sections
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