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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
FastDigest SchI
- 供应商：
上海研卉
- 规格：
100 reactions
描述
| 5' | G | A | G | T | C | N5 | ↓ | 3' | |||
| 3' | C | T | C | A | G | N5 | ↑ | 5' |
Thermo Scientific FastDigest SchI restriction enzyme recognizes GAGTC(5/5)^ site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: MlyI, PleI, PpsI, SchI.
Thermo Scientific FastDigest SchI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
规格
| Compatible Buffer: | 10x FastDigest Buffer/FastDigest Green Buffer |
|---|---|
| Enzyme: | SchI |
| Methylation Sensitivity: | Not CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive |
| Optimal Reaction Temperature: | 37° C |
| Product Line: | FastDigest |
| Product Size: | 100 reactions |
| Sensitive to Heat Inactivation: | Yes |
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文献和实验指由R.Feulgen和H.Rossenbeck（1924）提出的鉴定DNA的细胞化学反应。将细胞用 RNHCl在60℃下水解（水解时间由固定液而定）。如用Schi－ff′s试剂来作用，由于嘌呤碱基特异性的游离所生成的脱氧核糖的醛基与复红（fuchsin）反应，核染色体染成紫红色，表示DNA的存在。对由在最适条件基础上的富尔根反应所显色的DNA，可用显微分光测定法进行定量。
内切酶列表：Enzymes Generating Blunt Ends
^(5/5)GACTC* SchI GAGCGG(-3/-3)^* MbiI GAG^CTC Ecl136II GAGTC(5/5)^* SchI GAT^ATC Eco32
used for PCR in this protocol. 4. Cloning enzymes: FastDigest Bbs I, FastDigest Age I, FastAP(Thermo Scientifi c/Fermentas, Pittsburg, PA, USA), Plasmid-Safe exonuclease (Epicentre, Madison, WI, USA), T7 DNA ligase. 5. 10× T4 DNA ligase buffer
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