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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Hemopexin (Hpx)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Hemopexin (Hpx)品名简称:
Recombinant Rat Hpx protein货号:
CSB-EP010723RA规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
HpxUniprot No.:
P20059别名:
Hpx; Hemopexin种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
52.9 kDa表达区域:
24-460aa氨基酸序列:
NPLPAAHETVAKGENGTKPDSDVIEHCSDAWSFDATTMDHNGTMLFFKGEFVWRGHSGIRELISERWKNPVTSVDAAFRGPDSVFLIKEDKVWVYPPEKKENGYPKLFQEEFPGIPYPPDAAVECHRGECQSEGVLFFQGNRKWFWDFATRTQKERSWPAVGNCTAALRWLERYYCFQGNKFLRFNPVTGEVPPRYPLDARDYFISCPGRGHGKLRNGTAHGNSTHPMHSRCNADPGLSALLSDHRGATYAFSGSHYWRLDSSRDGWHSWPIAHHWPQGPSAVDAAFSWDEKVYLIQGTQVYVFLTKGGNNLVSGYPKRLEKELGSPPGISLDTIDAAFSCPGSSKLYVTSGRRLWWLDLKSGAQATWAELSWPHEKVDGALCLEKSLGPYSCSSNGPNLFFIHGPNLYCYSSIDKLNAAKSLPQPQKVNSILGCSQ蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Binds he and transports it to the liver for breakdown and iron recovery, after which the free hopexin returns to the circulation.功能2:
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.文献:
Rat hemopexin. Molecular cloning, primary structural characterization, and analysis of gene expression.Nikkilae H., Gitlin J.D., Mueller-Eberhard U.Biochemistry 30:823-829(1991)研究领域:
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文献和实验血红素结合蛋白(hemopexin,Hpx)分子量5.7万,单链多肽,含糖量约22%。正常血浆中含量为500-1000μg/L,和游离血红素有特异结合能力。它可配合结合珠蛋白对血红蛋白进行处理。当广泛溶血时,血浆结合珠蛋白耗竭,循环中游离的血红蛋白可降解为珠蛋白和血红素两部分。血红素不溶于水,可与Hpx结合成复合物而运输到肝,分子中的铁可被机体重新利用,卟啉环降解为胆红素而由胆管排出。Hpx并不能与血红蛋白结合,仅可与血红素可逆地结合,而在血循环中反复利用,这是机体有效地保
2-巨球蛋白、胆碱酯酶( cholinesterase)、碱性磷酸酶、乳酸脱氢酶。β部含有转铁蛋白、凝血素( hemopexin),补体、血清灭菌蛋白( properdin)、β 2-糖蛋白质、β 28-球蛋白、β 2r-球蛋白,γ部分含有免疫球蛋白( IgG, IgM, IgA, IgD, IgE等),红血球的内容物中,主要的蛋白质是血红蛋白,在各种动物的红血球中其干重(克%)是,人为 94.3,狗为 86.5,刺猬为 92.25,鹅为 62.65,蛇为 46.70。(血清球蛋白)。
直接被释放入血浆,与血浆中的结合珠蛋白(haptoglobin)�一种α2糖蛋白一结合,由于其分子较大,故不被肾脏排泄而被肝细胞摄取,最后变成胆红素。溶血较多时血浆中结合珠蛋白的浓度显著降低或消失,不过血浆中结合珠蛋白浓度的高低也受到其他多种因素的影响。 当血浆内结合珠蛋白全部与血红蛋白结合后,从游离血红蛋白分解出的血红素能与血结素(hemopexin)-一种β糖蛋白-结合,然后也被肝细胞摄取。大量溶血时血浆血结素的浓度亦降低。
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