Human Fibroblast Growth Factor

产品别名:HBGF6 ELISA Kit, HST2 ELISA Kit, HST2 Oncogene ELISA Kit, Heparin Secretory-Transforming Protein 2 ELISA Kit, Heparin-Binding Growth Factor 6 ELISA Kit

产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 6(FGF6).

应用说明：standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FGF6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FGF6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FGF6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FGF6 in the samples is then determined by comparing the OD of the samples to the standard curve

检测时间:3.5h

UniProt ID:P10767

Note:For research use only.