- ￥3510
- biorbyt
- 15.63-1000pg/mL
- 9.38 pg/mL
- orb1807846
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
15.63-1000pg/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
Tissue homogenate and Cell lysate
- 灵敏度：
9.38 pg/mL
- 规格：
48 T
产品别名：ECNOS, NOS Ⅲ, E-NOS
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS3/eNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NOS3/eNOS. You can calculate the concentration of Mouse NOS3/eNOS in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P70313
靶点：NOS3/eNOS
Note：For research use only.
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文献和实验-dependent and constitutively expressed and produce low levels of NO: NOS1 (neuronal NOS or nNOS), which is found mostly in neurons and skeletal muscle, and NOS3 (endothelial NOS or eNOS), which is found mostly in endothelial cells. NOS1 is critical
Isolated perfused lung (IPL) preparation from mouse expressing a FRET reporter ( protocol 1 ) 500 µM nitric oxide (NO) donor in perfusion buffer (see recipe ): e.g., dilute with perfusion
Assessment of Diabetic Nephropathy in the Akita Mouse
, endothelial nitric oxide synthase, or angiotensin-converting enzyme 2 leads to development of functionally and structurally more advanced diabetic nephropathy in these mice, while ketogenic diet has been shown to reverse kidney injury associated with diabetes
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